Rabbit anti-Human BRD3 Polyclonal Antibody | anti-BRD3 antibody

BRD3 (F645) Polyclonal Antibody

Cat. Num. AAA3002134

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BRD3; Polyclonal Antibody; BRD3 (F645) Polyclonal Antibody; Bromodomain-containing protein 3; RING3-like protein; KIAA0043; RING3L; anti-BRD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BRD3 (F645) polyclonal antibody detects endogenous levels of BRD3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 726

• Applicable Applications for anti-BRD3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 614-667 of Human BRD3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BRD3 pAb at 1:1000 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of BRD3 (F645) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-BRD3 antibody

The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomaincontaining proteins are ubiquitously expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 11067749
• NCBI Accession #: NP_031397.1
• NCBI GenBank Nucleotide #: NP_031397.1
• UniProt Accession #: Q15059
• Molecular Weight: ~72kDa
• NCBI Official Full Name: bromodomain-containing protein 3
• UniProt Protein Name: Bromodomain-containing protein 3
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD3
• UniProt Synonym Gene Names: KIAA0043; RING3L
• UniProt Entry Name: BRD3_HUMAN

Uniprot Description

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification