Western Blot (WB)
(Western Blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1:2000 dilution.)
Rabbit anti-Human BRD2 Polyclonal Antibody | anti-BRD2 antibody
BRD2 Polyclonal Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1:2000 dilution.)
Western Blot (WB)
(Western Blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1:2000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Immunocytochemistry (ICC)
(ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]
Research Articles on BRD2
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Product Notes
The BRD2 brd2 (Catalog #AAA3014174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRD2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the BRD2 brd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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