Rabbit anti-Human BRCC3/BRCC36 Polyclonal Antibody | anti-BRCC3/BRCC36 antibody

Anti-BRCC3/BRCC36 Antibody (aa2-316)

Cat. Num. AAA2401851

• Gene Names: BRCC3; C6.1A; BRCC36; CXorf53
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Caprylic acid and ammonium sulfate precipitation
• Synonyms: BRCC3/BRCC36; Polyclonal Antibody; Anti-BRCC3/BRCC36 Antibody (aa2-316); Rabbit Polyclonal (IgG) to Human BRCC3/BRCC36; BRCC3; C6.1A; BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; CXorf53; RP11-143H17.2; anti-BRCC3/BRCC36 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human BRCC3/BRCC36
• Purity/Purification: Caprylic acid and ammonium sulfate precipitation
• Form/Format: PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
• Sequence Length: 316

• Applicable Applications for anti-BRCC3/BRCC36 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 10 ug/ml
• Immunogen: Recombinant human Lys-63-specific deubiquitinase BRCC36 protein.
• Conjugation: Unconjugated
• Epitope: aa2-316
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

Western Blot (WB)

(Western blot of Lys-63-specific deubiquitinase BRCC36 antibody at 2 ug/ml with HeLa whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 36 KDa. Observed band size: 36 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

Related Product Information for anti-BRCC3/BRCC36 antibody

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitinchains. Does not have activity toward 'Lys-48'-linked polyubiquitinchains. Component of the BRCA1-A complex, a complex that specificallyrecognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNAlesions sites, leading to target the BRCA1-BARD1 heterodimer to sites ofDNA damage at double-str and breaks (DSBs).

NCBI and Uniprot Product Information

• NCBI GI #: 13236583
• NCBI GeneID: 79184
• NCBI Accession #: NP_077308.1
• NCBI GenBank Nucleotide #: NM_024332.3
• UniProt Accession #: P46736; Q16107; Q53YX5; Q9BTZ6; A6QRF8; A6QRF9; A8MUX5; A8MWH0; A9Z1Y0; A9Z1Y5; B1B062; B4DQN7
• Molecular Weight: 28,027 Da
• NCBI Official Full Name: lys-63-specific deubiquitinase BRCC36 isoform 1
• NCBI Official Synonym Full Names: BRCA1/BRCA2-containing complex subunit 3
• NCBI Official Symbol: BRCC3
• NCBI Official Synonym Symbols: C6.1A; BRCC36; CXorf53
• NCBI Protein Information: lys-63-specific deubiquitinase BRCC36
• UniProt Protein Name: Lys-63-specific deubiquitinase BRCC36
• UniProt Gene Name: BRCC3
• UniProt Synonym Gene Names: BRCC36; C6.1A; CXorf53

NCBI Description

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Uniprot Description

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).