Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human REST Polyclonal Antibody | anti-REST antibody
REST Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using REST antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
Uniprot Description
REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein; Cell development/differentiation
Chromosomal Location of Human Ortholog: 4q12
Cellular Component: nucleoplasm; transcriptional repressor complex; cytosol; nucleus
Molecular Function: protein binding; metal ion binding; outward rectifier potassium channel activity; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of insulin secretion; negative regulation of neurogenesis; negative regulation of cell proliferation; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; hemopoietic progenitor cell differentiation; negative regulation of transcription, DNA-dependent
Research Articles on REST
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Product Notes
The REST rest (Catalog #AAA9127275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REST Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's REST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the REST rest for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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