Rabbit anti-Human BRCC3 Polyclonal Antibody | anti-BRCC3 antibody

BRCC3 Antibody (N-Term)

Cat. Num. AAA9216570

• Gene Names: BRCC3; C6.1A; BRCC36; CXorf53
• Reactivity: Human
• Applications: Western Blot
• Synonyms: BRCC3; Polyclonal Antibody; BRCC3 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Lys-63-specific deubiquitinase BRCC36; 3419-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC36; C61A; CXorf53; anti-BRCC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This BRCC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 18-50 amino acids from human BRCC3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 291

• Applicable Applications for anti-BRCC3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-BRCC3 Antibody (N-Term) at 1:2000 dilutionLane 1: MCF-7 whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: human brain lysateLane 4: human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BRCC3 antibody

Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex.

Product Categories/Family for anti-BRCC3 antibody

Cancer; Cell biology

References

Kenwrick S.,et al.Hum. Mol. Genet. 1:179-186(1992). Fisch P.,et al.Oncogene 8:3271-3276(1993). Dong Y.,et al.Mol. Cell 12:1087-1099(2003). Ota T.,et al.Nat. Genet. 36:40-45(2004). Ross M.T.,et al.Nature 434:325-337(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 64762484
• NCBI GeneID: 79184
• NCBI Accession #: NP_001018065.1
• NCBI GenBank Nucleotide #: NM_001018055.2
• UniProt Accession #: P46736; Q16107; Q53YX5; Q9BTZ6; A6QRF8; A6QRF9; A8MUX5; A8MWH0; A9Z1Y0; A9Z1Y5; B1B062; B4DQN7
• Molecular Weight: 36072
• NCBI Official Full Name: lys-63-specific deubiquitinase BRCC36 isoform 2
• NCBI Official Synonym Full Names: BRCA1/BRCA2-containing complex subunit 3
• NCBI Official Symbol: BRCC3
• NCBI Official Synonym Symbols: C6.1A; BRCC36; CXorf53
• NCBI Protein Information: lys-63-specific deubiquitinase BRCC36
• UniProt Protein Name: Lys-63-specific deubiquitinase BRCC36
• Protein Family: Lys-63-specific deubiquitinase
• UniProt Gene Name: BRCC3
• UniProt Synonym Gene Names: BRCC36; C6.1A; CXorf53
• UniProt Entry Name: BRCC3_HUMAN

NCBI Description

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Uniprot Description

BRCC3: Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Ubiquitin conjugating system; Oncoprotein; EC 3.4.19.-

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity

Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; response to X-ray