Rabbit BPNT1 Polyclonal Antibody | anti-BPNT1 antibody

BPNT1 Rabbit Polyclonal

Cat. Num. AAA767171

• Gene Names: BPNT1; PIP; HEL20
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: BPNT1; Polyclonal Antibody; BPNT1 Rabbit Polyclonal; anti-BPNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 325

• Applicable Applications for anti-BPNT1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: 3'(2'), 5'-bisphosphate nucleotidase 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS767171 (BPNT1 antibody) at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HeLa cells using MBS767171 (BPNT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L))

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS767171 (BPNT1 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 17389533
• NCBI GeneID: 10380
• Molecular Weight: 29,189 Da
• NCBI Official Full Name: BPNT1 protein
• NCBI Official Synonym Full Names: 3'(2'), 5'-bisphosphate nucleotidase 1
• NCBI Official Symbol: BPNT1
• NCBI Official Synonym Symbols: PIP; HEL20
• NCBI Protein Information: 3'(2'),5'-bisphosphate nucleotidase 1
• UniProt Protein Name: 3'(2'),5'-bisphosphate nucleotidase 1
• UniProt Gene Name: BPNT1
• UniProt Synonym Gene Names: PIP
• UniProt Entry Name: BPNT1_HUMAN

NCBI Description

BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]

Uniprot Description

BPNT1: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4- trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.7; Phosphatase (non-protein); Motility/polarity/chemotaxis; Energy Metabolism - sulfur

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; cytosol

Molecular Function: 3'(2'),5'-bisphosphate nucleotidase activity; inositol-1,4-bisphosphate 1-phosphatase activity; magnesium ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; dephosphorylation; nervous system development; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; phosphoinositide phosphorylation