Rabbit MSI2 Polyclonal Antibody | anti-MSI2 antibody

MSI2 Antibody

Cat. Num. AAA150550

• Gene Names: MSI2; MSI2H
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: MSI2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: MSI2; Polyclonal Antibody; MSI2 Antibody; MSI2H; RNA-binding protein Musashi homolog 2; Musashi-2; musashi homolog 2 (Drosophila); anti-MSI2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.
• Purity/Purification: MSI2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; MSI2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 328

• Applicable Applications for anti-MSI2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: MSI2 antibody can be used for detection of MSI2 by Western blot at 1 ug/mL. Antibodycan also be used for immunohistochemistry starting at 5 ug/mL andimmunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL.; ; Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mousesamples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples.; ; All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2.; The immunogen is located within amino acids 30 - 80 of MSI2.
• Positive Control: MBS151675 - EL4 Cell Lysate; MBS154295 - EL4 Cell Slide
• Preparation and Storage: MSI2 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(MSI2 Antibody)

Immunocytochemistry (IHC)

(Immunocytochemistry of MSI2 in EL4 cells with MSI2 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of MSI2 in EL4 cells with MSI2 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MSI2 in mouse brain tissue with MSI2 Antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of MSI2 in mouse brain tissue with MSI2 Antibody at 20 μg/mL.Green: MSI2 antibody (6309)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-MSI2 antibody

MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.

NCBI and Uniprot Product Information

• NCBI GI #: 20373175
• NCBI GeneID: 124540
• NCBI Accession #: EAW94506
• NCBI GenBank Nucleotide #: NM_138962.2
• UniProt Accession #: Q96DH6; Q7Z6M7; Q8N9T4
• NCBI Official Full Name: RNA-binding protein Musashi homolog 2 isoform a
• NCBI Official Synonym Full Names: musashi RNA-binding protein 2
• NCBI Official Symbol: MSI2
• NCBI Official Synonym Symbols: MSI2H
• NCBI Protein Information: RNA-binding protein Musashi homolog 2; musashi-2; musashi homolog 2
• UniProt Protein Name: RNA-binding protein Musashi homolog 2
• Protein Family: RNA-binding protein
• UniProt Gene Name: MSI2
• UniProt Synonym Gene Names: Musashi-2
• UniProt Entry Name: MSI2H_HUMAN

NCBI Description

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Musashi-2: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). Belongs to the Musashi family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: polysome; cytoplasm

Molecular Function: nucleotide binding; poly(U) binding

Biological Process: stem cell development