Rabbit anti-Human BPGM Polyclonal Antibody | anti-Bpgm antibody

BPGM, CT (BPGM, Bisphosphoglycerate mutase, 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate synthase, 2,3-diphosphoglycerate mutase, BPG-dependent PGAM)

Cat. Num. AAA645558

• Gene Names: Bpgm; C86192; AI323730; AL022789
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: BPGM; Polyclonal Antibody; CT (BPGM; Bisphosphoglycerate mutase; 2; 3-bisphosphoglycerate mutase; erythrocyte; 3-bisphosphoglycerate synthase; 3-diphosphoglycerate mutase; BPG-dependent PGAM); Anti -BPGM; anti-Bpgm antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Bpgm antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-259 amino acids from the C-terminal region of human BPGM.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using MBS645558 (1:2000) Lane 1: MDA-MB-453 whole cell lysate 20ug/lane Lane 2: Hela whole cell lysate 20ug/lane Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 30kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Bpgm antibody

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Categories/Family for anti-Bpgm antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 6680806
• NCBI GeneID: 12183
• NCBI Accession #: NP_031589.1
• NCBI GenBank Nucleotide #: NM_007563.4
• UniProt Accession #: P15327; Q543Z6
• Molecular Weight: 29,978 Da
• NCBI Official Full Name: bisphosphoglycerate mutase
• NCBI Official Synonym Full Names: 2,3-bisphosphoglycerate mutase
• NCBI Official Symbol: Bpgm
• NCBI Official Synonym Symbols: C86192; AI323730; AL022789
• NCBI Protein Information: bisphosphoglycerate mutase; BPG-dependent PGAM; 2,3-bisphosphoglycerate synthase; 2,3-bisphosphoglycerate mutase, erythrocyte
• UniProt Protein Name: Bisphosphoglycerate mutase
• Protein Family: Bisphosphoglycerate mutase
• UniProt Gene Name: Bpgm
• UniProt Synonym Gene Names: BPGM
• UniProt Entry Name: PMGE_MOUSE

Uniprot Description

Function: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities.

Catalytic activity: 3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate.2-phospho-D-glycerate = 3-phospho-D-glycerate.2,3-bisphospho-D-glycerate + H2O = 3-phospho-D-glycerate + phosphate.

Enzyme regulation: At alkaline pH BPGM favors the synthase reaction; however, at lower pH the phosphatase reaction is dominant. Inhibited by citrate

By similarity.

Subunit structure: Homodimer.

Tissue specificity: Expressed in red blood cells. Expressed in placenta (labyrinthine trophoblasts). Ref.1 Ref.4

Sequence similarities: Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.