Rabbit anti-Human BNIP3L Polyclonal Antibody | anti-BNIP3L antibody

BNIP3L (BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3-like, Adenovirus E1B19K-Binding Protein B5, BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3A, NIP3-like Protein X, NIP3L, BNIP3A, BNIP3H, NIX) (Biotin)

Cat. Num. AAA6371378

• Gene Names: BNIP3L; NIX; BNIP3a
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BNIP3L; Polyclonal Antibody; BNIP3L (BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3-like; Adenovirus E1B19K-Binding Protein B5; BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3A; NIP3-like Protein X; NIP3L; BNIP3A; BNIP3H; NIX) (Biotin); anti-BNIP3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BNIP3L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BNIP3L antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human BNIP3L, aa1-219 (NP_004322.1).
• Immunogen Sequence: MSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLKVFIPSLFLSHVLALGLGIYIGKRLSTPSASTY
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of BNIP3L expression in transfected 293T cell line by BNIP3L polyclonal antibody. Lane 1: BNIP3L transfected lysate (23.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-BNIP3L antibody

Bcl-2/adenovirus E1B 19kD protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219aa, 24kD (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% aa sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.

Product Categories/Family for anti-BNIP3L antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4757860
• NCBI GeneID: 665
• NCBI Accession #: NP_004322.1
• NCBI GenBank Nucleotide #: NM_004331
• UniProt Accession #: O60238; Q5JW63; Q8NF87; Q6IBV1; Q9H3K3
• Molecular Weight: 23,930 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• NCBI Official Symbol: BNIP3L
• NCBI Official Synonym Symbols: NIX; BNIP3a
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; NIP3L; OTTHUMP00000125280; OTTHUMP00000225446; OTTHUMP00000225447; OTTHUMP00000225448; OTTHUMP00000225568; NIP3-like protein X; adenovirus E1B19k-binding protein B5; BCL2/adenovirus E1B 19-kd
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3L
• UniProt Synonym Gene Names: BNIP3A; BNIP3H; NIX; NIP3L
• UniProt Entry Name: BNI3L_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein counteracts the apoptotic inducer BNIP3 and may play a role in tumor suppression. [provided by RefSeq]

Uniprot Description

BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.

Protein type: Apoptosis; Membrane protein, integral; Mitochondrial; Endoplasmic reticulum; Nuclear envelope

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; integral to membrane; nuclear envelope; intrinsic to membrane

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding

Biological Process: viral reproduction; positive regulation of apoptosis; defense response to virus; negative regulation of apoptosis