Goat anti-Human BNIP3 Polyclonal Antibody | anti-BNIP3 antibody

BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

Cat. Num. AAA611920

• Gene Names: BNIP3; NIP3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BNIP3; Polyclonal Antibody; BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3; 19kD Interacting Protein 3; NIP3); Anti -BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3; anti-BNIP3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BNIP3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol, 0.02% SODIUM AZIDE.

• Applicable Applications for anti-BNIP3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human BCL2/adenovirus E1B 19kD protein-interacting protein 3 (aa2-160) SwissProt # Q12983
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human BNIP3 by Western Blot.)

Related Product Information for anti-BNIP3 antibody

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3), also known as 19kD interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5kD (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19kD protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Product Categories/Family for anti-BNIP3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 7669481
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.2
• NCBI GenBank Nucleotide #: NM_004052.2
• UniProt Accession #: Q12983; O14620; Q96GP0; Q53HF4; Q66K24; Q6NVY4
• Molecular Weight: 21,541 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; OTTHUMP00000020752
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BNIP3
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytoplasm; mitochondrial membrane; dendrite; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; neuron apoptosis; positive regulation of programmed cell death; response to hyperoxia; response to hypoxia; brown fat cell differentiation; autophagic cell death; mitochondrial fragmentation during apoptosis; regulation of mitochondrial membrane permeability; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis