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Western Blot (WB) ((0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat BNIP1 Polyclonal Antibody | anti-BNIP1 antibody

Goat anti-BNIP1 Antibody

Gene Names
BNIP1; NIP1; SEC20; TRG-8
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BNIP1; Polyclonal Antibody; Goat anti-BNIP1 Antibody; BCL2/adenovirus E1B 19kDa interacting protein 1; NIP1; SEC20; TRG-8; BCL2/adenovirus E1B 19kD interacting protein 1; BCL2/adenovirus E1B 19kD-interacting protein 1; BNIP1 antibody; BCL2/adenovirus E1B 19kDa interacting protein 1 antibody; NIP1 antibody; SEC20 antibody; TRG-8 antibody; BCL2/adenovirus E1B 19kD interacting protein 1 antibody; BCL2/adenovirus E1B 19kD-interacting protein 1 antibody; anti-BNIP1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DLLRQRKTTKESLAQ
Sequence Length
228
Applicable Applications for anti-BNIP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunogen
Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1.
Epitope
Internal region
Note
All reported variants represent isoform 1 (NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
662
NCBI GenBank Nucleotide #
Molecular Weight
27,333 Da
NCBI Official Full Name
BCL2/adenovirus E1B 19kD interacting protein 1 isoform BNIP1
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 1
NCBI Official Symbol
BNIP1
NCBI Official Synonym Symbols
NIP1; SEC20; TRG-8
NCBI Protein Information
vesicle transport protein SEC20
UniProt Protein Name
Vesicle transport protein SEC20
Protein Family
UniProt Gene Name
BNIP1
UniProt Synonym Gene Names
NIP1; SEC20L; TRG-8
UniProt Entry Name
SEC20_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq, Mar 2011]

Uniprot Description

BNIP1: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy. Belongs to the SEC20 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33-q34

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; nuclear envelope; SNARE complex

Molecular Function: protein binding; SNAP receptor activity

Biological Process: apoptosis; autophagy; endoplasmic reticulum membrane fusion; endoplasmic reticulum organization and biogenesis; negative regulation of apoptosis; retrograde vesicle-mediated transport, Golgi to ER

Research Articles on BNIP1

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Product Notes

The BNIP1 bnip1 (Catalog #AAA421283) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BNIP1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BNIP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Researchers should empirically determine the suitability of the BNIP1 bnip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DLLRQRKTTK ESLAQ. It is sometimes possible for the material contained within the vial of "BNIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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