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Western Blot (WB) ((0.1ug/ml) staining of rat stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat BMYB/MYBL2 Polyclonal Antibody | anti-MYBL2 antibody

Goat anti-BMYB/MYBL2 Antibody

Gene Names
MYBL2; BMYB; B-MYB
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BMYB/MYBL2; Polyclonal Antibody; Goat anti-BMYB/MYBL2 Antibody; MYBL2; BMYB; v-myb myeloblastosis viral oncogene homolog (avian)-like 2; HGNC: 7548; MGC15600; MYB-related protein B; v-myb avian myeloblastosis viral oncogene homolog-like 2; B-MYB; MYBL2 antibody; BMYB antibody; v-myb myeloblastosis viral oncogene homolog (avian)-like 2 antibody; HGNC: 7548 antibody; MGC15600 antibody; MYB-related protein B antibody; v-myb avian myeloblastosis viral oncogene homolog-like 2 antibody; B-MYB antibody; BMYB / MYBL2; anti-MYBL2 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QEKARQLLGRLKPSH
Sequence Length
700
Applicable Applications for anti-MYBL2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 100kDa band observed in rat stomach lysates (calculated MW of 78.8kDa according to Human NP_002457.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-QEKARQLLGRLKPSH, from the C Terminus of the protein sequence according to NP_002457.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of rat stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of rat stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,865 Da
NCBI Official Full Name
myb-related protein B isoform 1
NCBI Official Synonym Full Names
MYB proto-oncogene like 2
NCBI Official Symbol
MYBL2
NCBI Official Synonym Symbols
BMYB; B-MYB
NCBI Protein Information
myb-related protein B
UniProt Protein Name
Myb-related protein B
Protein Family
UniProt Gene Name
MYBL2
UniProt Synonym Gene Names
BMYB; B-Myb
UniProt Entry Name
MYBB_HUMAN

NCBI Description

The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

B-Myb: a transcription factor involved in cell cycle progression. Phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm

Molecular Function: protein binding; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; transcription from RNA polymerase II promoter

Research Articles on MYBL2

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Product Notes

The MYBL2 mybl2 (Catalog #AAA421249) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BMYB/MYBL2 Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's BMYB/MYBL2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 100kDa band observed in rat stomach lysates (calculated MW of 78.8kDa according to Human NP_002457.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the MYBL2 mybl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QEKARQLLGR LKPSH. It is sometimes possible for the material contained within the vial of "BMYB/MYBL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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