Rabbit anti-Human Bmp7 Polyclonal Antibody | anti-Bmp7 antibody

Bmp7, NT (Bone Morphogenetic Protein 7, BMP-7, Osteogenic Protein 1, OP-1, Eptotermin alfa, OP1) (Biotin)

Cat. Num. AAA6465574

• Gene Names: BMP7; OP-1
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bmp7; Polyclonal Antibody; NT (Bone Morphogenetic Protein 7; BMP-7; Osteogenic Protein 1; OP-1; Eptotermin alfa; OP1) (Biotin); anti-Bmp7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bmp7.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Bmp7 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:50-1:100; ELISA: 1:1,000; IHC: 1:50-1:100; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Bmp7 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Bmp7 (arrow) using rabbit polyclonal Bmp7 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Bmp7 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Bmp7 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Product Categories/Family for anti-Bmp7 antibody

Antibodies; Growth Factors; Cytokines

References

1. Merrihew, C., et al., J. Orthop. Res. 21(5):899-907 (2003). 2. Greenwald, J., et al., Mol. Cell 11(3):605-617 (2003). 3. Maric, I., et al., J. Cell. Physiol. 196(2):258-264 (2003). 4. Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003). 5. Lee, M.J., et al., J. Immunol. 170(5):2557-2563 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4502427
• NCBI GeneID: 655
• NCBI Accession #: NP_001710
• NCBI GenBank Nucleotide #: NM_001719.2
• UniProt Accession #: P18075; Q9H512; Q9NTQ7
• NCBI Official Full Name: bone morphogenetic protein 7 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone, kidney and brown adipose tissue development. Additionally, this protein induces ectopic bone formation and may promote fracture healing in human patients. [provided by RefSeq, Jul 2016]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: negative regulation of MAP kinase activity; axon guidance; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; BMP signaling pathway; regulation of apoptosis; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; epithelial to mesenchymal transition; negative regulation of phosphorylation; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth