Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using BMP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human BMP4 Polyclonal Antibody | anti-BMP4 antibody
BMP4 Rabbit pab Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
BMP4; Polyclonal Antibody; BMP4 Rabbit pab Antibody; BMP4 ; BMP2B ; BMP2B1 ; MCOPS6; OFC11; ZYME ; Bone morphogenetic protein 4 ; Bone morphogenetic protein 2B;; anti-BMP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
408
Applicable Applications for anti-BMP4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunoprecipitation (IP): 1/10-50
Flow Cytometry (FC/FACS): 1/50-100
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunoprecipitation (IP): 1/10-50
Flow Cytometry (FC/FACS): 1/50-100
Immunogen
A synthetic -peptide of human BMP4
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using BMP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using BMP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-BMP4 antibody
Rabbit polyclonal to Human BMP4
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-beta superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP4 plays an essential role during development. Mouse embyros deficient in BMP4 die around the time of gastrulation (5). BMP4 regulates stem cell maintenance and differentiation in various systems (6-8).
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-beta superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4). BMP4 plays an essential role during development. Mouse embyros deficient in BMP4 die around the time of gastrulation (5). BMP4 regulates stem cell maintenance and differentiation in various systems (6-8).
References
1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8. 2. Wozney, J.M. et al. (1988) Science 242, 1528-34. 3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61. 4. Nohe, A. et al. (2004) Cell Signal 16, 291-9. 5. Dunn, N.R. et al. (1997) Dev Biol 188, 235-47. 6. Nakayama, N. et al. (2000) Blood 95, 2275-83. 7. Solmesky, L.J. et al. (2009) Stem Cells Dev 18, 1283-92. 8. Goldman, O. et al. (2009) Stem Cells 27, 1750-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
408
NCBI Official Full Name
bone morphogenetic protein 4 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 4
NCBI Official Symbol
BMP4
NCBI Official Synonym Symbols
ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
NCBI Protein Information
bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B
UniProt Protein Name
Bone morphogenetic protein 4
Protein Family
UniProt Gene Name
BMP4
UniProt Synonym Gene Names
BMP2B; DVR4; BMP-4; BMP-2B
UniProt Entry Name
BMP4_HUMAN
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Product Notes
The BMP4 bmp4 (Catalog #AAA125763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BMP4 Rabbit pab Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1/1000-2000 Immunohistochemistry (IHC): 1/50-100 Immunocytochemistry (ICC): 1/50-100 Immunoprecipitation (IP): 1/10-50 Flow Cytometry (FC/FACS): 1/50-100. Researchers should empirically determine the suitability of the BMP4 bmp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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