BMP-2 Polyclonal Antibody | anti-BMP-2 antibody

Rabbit anti BMP-2 Polyclonal

Cat. Num. AAA551015

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: >95%. Serum IgG fraction was purified by Protein G affinity chromatography.
• Synonyms: BMP-2; Polyclonal Antibody; Rabbit anti BMP-2 Polyclonal; Rabbit anti BMP-2 Polyclonal purified; bmp2; bone morphogenetic protein; bmp-2; pab; polyclonal; anti-BMP-2 antibody

• Host: Polyclonal anti BMP-2 was produced from sera of rabbits immunized with synthetic peptide containing BMP-2 C-terminus antigenic determinants.
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%. Serum IgG fraction was purified by Protein G affinity chromatography.
• Form/Format: 0.2um filtered 1mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin.
• Sequence Length: 396

• Applicable Applications for anti-BMP-2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: Use at a concentration range of 1-2ug/ml.; Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
• Source Note: Polyclonal BMP-2 antibody was produced from sera of rabbits immunized with synthetic protein containing BMP-2 antigenic determinants.
• Reconstitution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.
• Preparation and Storage: This antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for 12 months without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for anti-BMP-2 antibody

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP-2 wild type binds to its cellular receptors via two distinct binding epitopes. The large epitope 1 is responsible for the high- affinity binding to the BMPR-IA receptor, the smaller epitope 2 provides the low-affinity binding to the receptor BMPR-II.

Product Categories/Family for anti-BMP-2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.2
• UniProt Accession #: P12643
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: BMP-2-inducible protein kinase
• UniProt Gene Name: BMP2
• UniProt Synonym Gene Names: BMP2A; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Induces cartilage and bone formation.

Subunit structure: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN

By similarity. Ref.4

Subcellular location: Secreted.

Tissue specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Pharmaceutical use: Available under the name Infuse (Medtronic Sofamor Danek). Used for treating open tibial shaft fractures.

Sequence similarities: Belongs to the TGF-beta family.