Rabbit anti-Human, Mouse BLVRB Polyclonal Antibody | anti-BLVRB antibody

BLVRB Antibody

Cat. Num. AAA9417412

• Gene Names: BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: BLVRB; Polyclonal Antibody; BLVRB Antibody; FLR; BVRB; SDR43U1; HEL-S-10; anti-BLVRB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total BLVRB protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1.6mg/ml (varies by lot)
• Sequence Length: 206

• Applicable Applications for anti-BLVRB antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot: 1:500-2000; Immunohistochemistry: 1: 100-200
• Immunogen: Synthetic peptide of human BLVRB
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C

SDS-Page

(Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-2: A549 and K562 cell lysate, Primary antibody:BLVRB antibody at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using BLVRB Antibody at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: x200))

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BLVRB Antibody at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: x200))

Related Product Information for anti-BLVRB antibody

BLVRB (biliverdin reductase B or BVR-B), also known as flavin reductase (FR), NADPH-dependent diaphorase, Biliverdin-IX ¡'-reductase or green heme-binding protien (GHBP) is an enzyme involved in fetal heme metabolism. It is dependent on NADPH and is responsible for catalyzing the transfer of electrons to flavins from reduced pyridine nucleotides. BLVRB exists as a monomer, localizes to the cytoplasm and is highly expressed in fetal liver and adult erythrocytes and, to a lesser extent, in heart, lung, cerebrum and adrenal gland. In liver, BLVRB functions to convert biliverdin (isoforms IX¡', IX? and IX?) to bilirubin. BLVRB contains one binding site for all of its substrates and predominantly interacts with them through hydrophobic interactions. BLVRB also exhibits ferric reductase activity. In addition, it is commonly used as a reliable marker for NOS.

Product Categories/Family for anti-BLVRB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502419
• NCBI GeneID: 645
• NCBI Accession #: NP_000704.1
• NCBI GenBank Nucleotide #: NM_000713.2
• UniProt Accession #: P30043; P32078; P53005; Q32LZ2; A6NKD8; B2R5C6
• Molecular Weight: 22,119 Da
• NCBI Official Full Name: flavin reductase (NADPH)
• NCBI Official Synonym Full Names: biliverdin reductase B
• NCBI Official Symbol: BLVRB
• NCBI Official Synonym Symbols: FLR; BVRB; SDR43U1; HEL-S-10
• NCBI Protein Information: flavin reductase (NADPH)
• UniProt Protein Name: Flavin reductase (NADPH)
• UniProt Gene Name: BLVRB
• UniProt Synonym Gene Names: FLR; BVR-B; GHBP; FLR
• UniProt Entry Name: BLVRB_HUMAN

NCBI Description

The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]

Uniprot Description

BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.3.1.24; EC 1.5.1.30; Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane

Molecular Function: biliverdin reductase activity; flavin reductase activity

Biological Process: heme catabolic process