Rabbit Blimp-1 Polyclonal Antibody | anti-Blimp-1 antibody

Blimp-1 Antibody

Cat. Num. AAA9613082

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (92%), Horse (83%), Sheep (92%), Rabbit (100%), Dog (92%)
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Blimp-1; Polyclonal Antibody; Blimp-1 Antibody; B Lymphocyte Induced Maturation Protein 1; Beta interferon gene positive regulatory domain I binding factor; Beta-interferon gene positive regulatory domain I-binding factor; BLIMP-1; BLIMP1; Positive Regulatory Domain I Binding Factor 1; Positive regulatory domain I-binding factor 1; PR Domain Containing 1; PR domain containing 1 with ZNF domain; PR domain containing 1 with ZNF domain isoform 2; PR domain containing protein 1; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI binding factor 1; PRDI-BF1; PRDI-binding factor 1; PRDM 1; Prdm1; PRDM1_HUMAN; anti-Blimp-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (92%), Horse (83%), Sheep (92%), Rabbit (100%), Dog (92%)
• Clonality: Polyclonal
• Isotype: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Specificity: Blimp-1 Antibody detects endogenous levels of total Blimp-1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Blimp-1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-400; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Blimp-1, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.Ubiquitinated by the SCF (FBXO11) complex, leading to its degradation by the proteasome.
• Subunit Structure: Interacts with PRMT5 (By similarity). Interacts with FBXO10. Interacts with FBXO11.
• Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily.
• Subcellular Location: Nucleus. Cytoplasm.
• Preparation and Storage: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Western Blot (WB)

(Western blot analysis of extracts from JK and Raji cells using Blimp-1 Antibody)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Staining HEPG2 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Related Product Information for anti-Blimp-1 antibody

Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity).
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. NANOS3 function in human germ cell development.

NCBI and Uniprot Product Information

• NCBI GI #: 172072684
• NCBI GeneID: 639
• NCBI Accession #: NP_001189.2
• NCBI GenBank Nucleotide #: NM_001198.3
• UniProt Accession #: O75626; Q86WM7; B2REA6; E1P5E0
• Molecular Weight: 825
• NCBI Official Full Name: PR domain zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1; BLIMP-1; PRDI-binding factor-1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive-regulatory domain I binding factor
• UniProt Protein Name: PR domain zinc finger protein 1
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1
• UniProt Entry Name: PRDM1_HUMAN