Rabbit Blimp-1 Polyclonal Antibody | anti-Blimp-1 antibody

Blimp-1 Antibody

Cat. Num. AAA9412145

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Blimp-1; Polyclonal Antibody; Blimp-1 Antibody; B-lymphocyte-induced maturation protein 1; PR domain-containing protein 1; PRDI-binding factor 1; PRDI-BF1; anti-Blimp-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 825

• Applicable Applications for anti-Blimp-1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide from near the amino terminus of human Blimp-1.
• Target Name: Blimp-1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 5 ug/mL.)

Related Product Information for anti-Blimp-1 antibody

Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells. Blimp-1 exists as at least two different isoforms.

Product Categories/Family for anti-Blimp-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 172072684
• NCBI GeneID: 639
• NCBI Accession #: NP_001189
• NCBI GenBank Nucleotide #: NM_001198.3
• Molecular Weight: 76,835 Da
• NCBI Official Full Name: PR domain zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1
• UniProt Protein Name: PR domain zinc finger protein 1
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1; PRDI-binding factor 1
• UniProt Entry Name: PRDM1_HUMAN

NCBI Description

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; DNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; protein binding; histone deacetylase binding; metal ion binding; transcription factor activity

Biological Process: methylation; negative regulation of lipopolysaccharide-mediated signaling pathway; eye photoreceptor cell development; cell fate commitment; transcription, DNA-dependent; positive regulation of B cell differentiation; negative regulation of B cell proliferation; negative regulation of transcription from RNA polymerase II promoter; maternal placenta development; post-embryonic development; germ cell development