Rabbit anti-Human, Mouse BIN1 Polyclonal Antibody | anti-BIN1 antibody

BIN1, ID (BIN1, AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) (APC)

Cat. Num. AAA6278418

• Gene Names: BIN1; CNM2; AMPH2; AMPHL; SH3P9
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BIN1; Polyclonal Antibody; ID (BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1) (APC); Bridging integrator 1; anti-BIN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 506

• Applicable Applications for anti-BIN1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-389 amino acids from the Central region of human BIN1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BIN1 Antibody (Center) western blot analysis in L929 cell line lysates (35ug/lane).This demonstrates the BIN1 antibody detected the BIN1 protein (arrow).)

Western Blot (WB)

(BIN1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BIN1 antibody detected the BIN1 protein (arrow).)

Immunohistochemistry (IHC)

(BIN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BIN1 antibody

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Product Categories/Family for anti-BIN1 antibody

Antibodies; Cardiac Markers

References

Biffi, A., et al. Arch. Neurol. 67(6):677-685(2010). Seshadri, S., et al. JAMA 303(18):1832-1840(2010). Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Claeys, K.G., et al. Neurology 74(6):519-521(2010). Hong, T.T., et al. PLoS Biol. 8 (2), E1000312 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 21536404
• NCBI GeneID: 274
• NCBI Accession #: NP_647595.1; NP_647594.1; NP_004296.1
• NCBI GenBank Nucleotide #: NP_647595.1
• UniProt Accession #: O00499
• NCBI Official Full Name: myc box-dependent-interacting protein 1 isoform 3
• NCBI Official Synonym Full Names: bridging integrator 1
• NCBI Official Symbol: BIN1
• NCBI Official Synonym Symbols: CNM2; AMPH2; AMPHL; SH3P9
• NCBI Protein Information: myc box-dependent-interacting protein 1
• UniProt Protein Name: Myc box-dependent-interacting protein 1
• Protein Family: Myc box-dependent-interacting protein
• UniProt Gene Name: BIN1
• UniProt Synonym Gene Names: AMPHL
• UniProt Entry Name: BIN1_HUMAN

NCBI Description

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Mar 2016]

Uniprot Description

BIN1: May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. Defects in BIN1 are the cause of centronuclear myopathy type 2 (CNM2). A congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Cytoskeletal; Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: I band; synaptic vesicle; membrane; axon; T-tubule; cytoplasm; nerve terminal; Z disc; nucleus; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; GTPase binding; protein heterodimerization activity; protein complex binding; tau protein binding

Biological Process: cell proliferation; muscle cell differentiation; viral reproduction; regulation of neuron differentiation; positive regulation of apoptosis; positive regulation of endocytosis; endocytosis; positive regulation of GTPase activity; positive regulation of astrocyte differentiation

Disease: Myopathy, Centronuclear, 2