Western Blot (WB)
(Western blot analysis of Bid expression in Jurkat cell lysate.)
Rabbit Bid Polyclonal Antibody | anti-BID antibody
Bid Antibody
Gene Names
BID; FP497
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Chromatography
Synonyms
Bid; Polyclonal Antibody; Bid Antibody; Rabbit Polyclonal Bid Antibody; Polyclonal Bid antibody; anti-Bid antibody; MGC15319 antibody; MGC42355 antibody; FP497 antibody; anti-BID antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% Glycerol, 0.5% BSA, 0.01% Thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
195
Applicable Applications for anti-BID antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 5-10 ug/ml
IHC: 20-40 ug/ml
WB: 0.5-4 ug/ml
IHC: 20-40 ug/ml
WB: 0.5-4 ug/ml
Immunogen
Bid antibody was raised in Rabbit using synthetic peptide surrounding amino acid 37 of human Bid as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Bid expression in Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of Bid expression in Jurkat cell lysate.)
Related Product Information for anti-BID antibody
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Product Categories/Family for anti-BID antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
BID
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
BID
NCBI Official Synonym Symbols
FP497
NCBI Protein Information
BH3-interacting domain death agonist
Protein Family
NCBI Description
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
Research Articles on BID
Similar Products
Product Notes
The BID (Catalog #AAA5311204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bid can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 5-10 ug/ml IHC: 20-40 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the BID for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bid, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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