Goat BID Polyclonal Antibody | anti-BID antibody

GOAT ANTI HUMAN BID (C-TERMINAL)

Cat. Num. AAA221364

• Gene Names: BID; FP497
• Applications: ELISA, Western Blot
• Synonyms: BID; Polyclonal Antibody; GOAT ANTI HUMAN BID (C-TERMINAL); anti-BID antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the C-terminus of BID, a death agonist that heterodimerizes with other members of the Bcl-2 family of cell death regulators; including the agonist BAX or antagonist BCL2. ; ; BID contains a BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Protease-cleaved BID translocates to mitochondria where it results in cytochrome c release, leading to caspase activation and cell death. This constitutes the activation of the mitochondria death pathway (the intrinsic pathway) by the death stimuli. ; ; BID has been shown to promote cell cycle progression into S phase, whilst BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. Deletion of BID inhibits carcinogenesis in the liver, and promotes tumorigenesis in the myeloid cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 99

• Applicable Applications for anti-BID antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects bands of approximately 26kDa and 24kDa in A431 cell lysates.; ELISA: Maximum Dilution: 1/8000; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.3ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Peptide with sequence C-TYVRSLARNGMD from the C-terminus of BID; Buffer Solution
• Antiserum Preparation: Antisera to BID (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-BID antibody

MBS221364 detects an epitope within the C-terminus of BID, a death agonist that heterodimerizes with other members of the Bcl-2 family of cell death regulators; including the agonist BAX or antagonist BCL2. BID contains a BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Protease-cleaved BID translocates to mitochondria where it results in cytochrome c release, leading to caspase activation and cell death. This constitutes the activation of the mitochondria death pathway (the intrinsic pathway) by the death stimuli. BID has been shown to promote cell cycle progression into S phase, whilst BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. Deletion of BID inhibits carcinogenesis in the liver, and promotes tumorigenesis in the myeloid cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557361
• NCBI GeneID: 637
• NCBI Accession #: NP_001187.1
• NCBI GenBank Nucleotide #: NM_001196.3
• UniProt Accession #: P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86
• Molecular Weight: 11,263 Da
• NCBI Official Full Name: BH3-interacting domain death agonist isoform 2
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; Human BID coding sequence; apoptic death agonist; desmocollin type 4; p22 BID
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; neuron apoptosis; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization