Aprataxin Recombinant Protein | APTX recombinant protein

Aprataxin, 1-168aa, Human, His tag, E. coli

Cat. Num. AAA203526

• Gene Names: APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Aprataxin; 1-168aa; Human; His tag; E. coli; APTX; AXA1; AOA; AOA 1; AOA1; Aprataxin homolog; Ataxia 1 early onset with hypoalbuminemia; Ataxia1 early onset with hypoalbuminemia; AXA 1; EAOH; EOAHA; FHA HIT; FLJ20157; MGC1072; Forkhead associated domain histidine triad like; Forkhead associated domain histidine triad like protein.; APTX recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 7.5) containing 20% glycerol, 100mM NaCl, 0.1mM PMSF
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKMQVYKDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHM HTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LESQAVIEMV QEAGRVTVRD GMPELLKLPL RCHECQQLLP SIPQLKEHLR KHWTQ
• Sequence Length: 192

• Applicable Applications for APTX recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for APTX recombinant protein

Aprataxin is a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. Aprataxin may play a role in single-stranded DNA repair. Mutations in the gene have been associated with neurological disorder known as ataxia oculomotor apraxia-1. Recombinant Aprataxin protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for APTX recombinant protein

Neuroscience

References

Kijas A.W., et al (2006) J. Biol. Chem. 281:13939-13948; Rass u., et al. (2007) J. Biol. Chem. 282:9469-9474

NCBI and Uniprot Product Information

• NCBI GI #: 28329427
• NCBI GeneID: 54840
• NCBI Accession #: NP_778241
• NCBI GenBank Nucleotide #: NM_175071.1
• Molecular Weight: 23.9kDa (205aa), confirmed by MALDI-TOF.
• NCBI Official Synonym Full Names: aprataxin
• NCBI Official Symbol: APTX
• NCBI Official Synonym Symbols: AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• NCBI Protein Information: aprataxin
• UniProt Protein Name: Aprataxin
• Protein Family: Aprataxin
• UniProt Gene Name: APTX
• UniProt Synonym Gene Names: AXA1; FHA-HIT

NCBI Description

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed:15380105, PubMed:15044383, PubMed:16964241, PubMed:17276982, PubMed:24362567). Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed:16964241, PubMed:24362567). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241, PubMed:17276982, PubMed:24362567). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH2) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (PubMed:16547001). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) ().