Rabbit BHC80/PHF21A Polyclonal Antibody | anti-BHC80/PHF21A antibody
Anti-BHC80/PHF21A Antibody
Gene Names
PHF21A; BHC80; BM-006
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Immunogen Affinity Purified
Synonyms
BHC80/PHF21A; Polyclonal Antibody; Anti-BHC80/PHF21A Antibody; PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006; anti-BHC80/PHF21A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
680
Applicable Applications for anti-BHC80/PHF21A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
Immunogen
E Coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
Relevant Detection Systems
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
Relevant Detection Systems
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-BHC80/PHF21A antibody
Description: Rabbit IgG polyclonal antibody for BHC80/PHF21A detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.
Background: PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).
Background: PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).
References
1. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347-55. 2. Iwase S, Januma A, Miyamoto K, Shono N, Honda A, Yanagisawa J, Baba T (Sep 2004). "Characterization of BHC80 in BRAF-HDAC complex, involved in neuron-specific gene repression". Biochem. Biophys. Res. Commun. 322 (2): 601-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
PHD finger protein 21A isoform a
NCBI Official Synonym Full Names
PHD finger protein 21A
NCBI Official Symbol
PHF21A
NCBI Official Synonym Symbols
BHC80; BM-006
NCBI Protein Information
PHD finger protein 21A
UniProt Protein Name
PHD finger protein 21A
UniProt Gene Name
PHF21A
UniProt Synonym Gene Names
BHC80; KIAA1696; BM-006
UniProt Entry Name
PF21A_HUMAN
NCBI Description
The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).[supplied by OMIM, Nov 2010]
Research Articles on BHC80/PHF21A
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Product Notes
The BHC80/PHF21A phf21a (Catalog #AAA1752545) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BHC80/PHF21A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BHC80/PHF21A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA). WB: 0.1-0.5ug/ml IHC-P: 0.5-1ug/ml Direct ELISA: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the BHC80/PHF21A phf21a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHC80/PHF21A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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