Rabbit anti-Human BGN Polyclonal Antibody | anti-BGN antibody

BGN, ID (Biglycan, Bone/Cartilage Proteoglycan I, PG-S1, SLRR1A)

Cat. Num. AAA624365

• Gene Names: BGN; PGI; DSPG1; PG-S1; SLRR1A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: BGN; Polyclonal Antibody; ID (Biglycan; Bone/Cartilage Proteoglycan I; PG-S1; SLRR1A); Anti -BGN; anti-BGN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BGN.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-BGN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human BGN (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BGN (arrow) using rabbit polyclonal BGN Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.)

Western Blot (WB)

(Western blot analysis of BGN Antibody (Center) in Sk-Br-3 cell line lysates (35ug/lane). BGN (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with BGN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BGN Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BGN antibody

BGN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival.

Product Categories/Family for anti-BGN antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 4502403
• NCBI GeneID: 633
• NCBI Accession #: NP_001702.1
• NCBI GenBank Nucleotide #: NM_001711.4
• UniProt Accession #: P21810; P13247; D3DWU3
• Molecular Weight: 41,654 Da
• NCBI Official Full Name: biglycan preproprotein
• NCBI Official Synonym Full Names: biglycan
• NCBI Official Symbol: BGN
• NCBI Official Synonym Symbols: PGI; DSPG1; PG-S1; SLRR1A
• NCBI Protein Information: biglycan; biglycan proteoglycan; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; small leucine-rich protein 1A; dermatan sulphate proteoglycan I
• UniProt Protein Name: Biglycan
• Protein Family: Biglycan
• UniProt Gene Name: BGN
• UniProt Synonym Gene Names: SLRR1A
• UniProt Entry Name: PGS1_HUMAN

NCBI Description

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009]

Uniprot Description

BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma

Molecular Function: glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent

Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; pathogenesis; blood vessel remodeling; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process