Human Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) ELISA Kit | PIN1 elisa kit

Human Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) ELISA Kit

Cat. Num. AAA2088178

• Gene Names: PIN1; DOD; UBL5
• Reactivity: Human
• Synonyms: Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1); Human Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) ELISA Kit; DOD; Protein(Peptidyl-Prolyl Cis/Trans Isomerase) NIMA-Interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; Rotamase Pin1; PIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1).; No significant cross-reactivity or interference between Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) and analogues was observed.
• Sequence Length: 163

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PIN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5453898
• NCBI GeneID: 5300
• NCBI Accession #: NP_006212.1
• NCBI GenBank Nucleotide #: NM_006221.3
• UniProt Accession #: Q13526; Q53X75; A8K4V9
• Molecular Weight: 18,243 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
• NCBI Official Synonym Full Names: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• NCBI Official Symbol: PIN1
• NCBI Official Synonym Symbols: DOD; UBL5
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PIN1
• UniProt Synonym Gene Names: PPIase Pin1

NCBI Description

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed:21497122, PubMed:22033920, PubMed:23623683). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:23623683, PubMed:27561354).