Rabbit OAT1 Polyclonal Antibody | anti-OAT1 antibody

OAT1 (L304) Polyclonal Antibody

Cat. Num. AAA3003928

• Gene Names: SLC22A6; OAT1; PAHT; HOAT1; ROAT1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: OAT1; Polyclonal Antibody; OAT1 (L304) Polyclonal Antibody; Solute carrier family 22 member 6; Organic anion transporter 1; hOAT1; PAH transporter; hPAHT; Renal organic anion transporter 1; hROAT1; SLC22A6; PAHT; anti-OAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OAT1 (L304) polyclonal antibody detects endogenous levels of OAT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 563

• Applicable Applications for anti-OAT1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 271-320 of Human OAT1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of OAT1 (L304) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-OAT1 antibody

OAT1 (organic anion transporter 1), a 563 amino acid protein, and OAT3 (organic anion transporter 3), a 542 amino acid protein, are two members of the OAT family and are highly expressed in kidneys. Localized specifically to the basolateral membrane, OAT1 and OAT3 are involved in the elimination of toxic organic anions, such as benzylpenicillin and cimetidine, from proximal renal tubules. Via their ability to remove anions from renal tissues, OAT1 and OAT3 are able to regulate the amount of toxins within the kidneys. Additionally, OAT1 functions as an organic anion exchanger that couples the uptake of one organic anion molecule with the efflux of one endogenous dicarboxylic acid molecule, such as ketoglutarate.

NCBI and Uniprot Product Information

• NCBI GI #: 20070188
• NCBI GeneID: 9356
• NCBI Accession #: NP_004781.2
• NCBI GenBank Nucleotide #: NP_004781.2
• UniProt Accession #: O95742; Q4U2R8
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: solute carrier family 22 member 6 isoform a
• NCBI Official Synonym Full Names: solute carrier family 22 member 6
• NCBI Official Symbol: SLC22A6
• NCBI Official Synonym Symbols: OAT1; PAHT; HOAT1; ROAT1
• NCBI Protein Information: solute carrier family 22 member 6
• UniProt Protein Name: Solute carrier family 22 member 6
• UniProt Gene Name: SLC22A6
• UniProt Synonym Gene Names: OAT1; PAHT; hOAT1; hPAHT; hROAT1
• UniProt Entry Name: S22A6_HUMAN

NCBI Description

The protein encoded by this gene is involved in the sodium-dependent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and may be localized to the basolateral membrane. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC22A6: Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS). Mediates the sodium-independent uptake of p- aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido- 3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro- phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2- furanpropionate (CMPF), cidofovir, adefovir, 9-(2- phosphonylmethoxyethyl) guanine (PMEG), 9-(2- phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p- chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid. PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: protein complex; integral to plasma membrane; basolateral plasma membrane; plasma membrane; caveola

Molecular Function: protein binding; protein homodimerization activity; inorganic anion exchanger activity; organic anion transmembrane transporter activity; sodium-independent organic anion transmembrane transporter activity; chloride ion binding

Biological Process: sodium-independent organic anion transport; alpha-ketoglutarate transport; response to methotrexate; transmembrane transport; protein homooligomerization; organic anion transport