Rabbit Bfl-1 Polyclonal Antibody | anti-Bfl-1 antibody

Bfl-1 Antibody

Cat. Num. AAA9405800

• Gene Names: BCL2A1; GRS; ACC1; ACC2; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Bfl-1; Polyclonal Antibody; Bfl-1 Antibody; Bcl-2-related protein A1; Hemopoietic-specific early response protein; anti-Bfl-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 175

• Applicable Applications for anti-Bfl-1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide from near the amino terminus of human Bfl-1.
• Target Name: Bfl-1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Bfl-1 in mouse kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 2 ug/mL.)

Related Product Information for anti-Bfl-1 antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.

Product Categories/Family for anti-Bfl-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4757840
• NCBI GeneID: 597
• NCBI Accession #: NP_004040
• NCBI GenBank Nucleotide #: NM_004049.3
• Molecular Weight: 18,746 Da
• NCBI Official Full Name: bcl-2-related protein A1 isoform 1
• NCBI Official Synonym Full Names: BCL2-related protein A1
• NCBI Official Symbol: BCL2A1
• NCBI Official Synonym Symbols: GRS; ACC1; ACC2; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• NCBI Protein Information: bcl-2-related protein A1
• UniProt Protein Name: Bcl-2-related protein A1
• UniProt Gene Name: BCL2A1
• UniProt Synonym Gene Names: BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5
• UniProt Entry Name: B2LA1_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: mitochondrial outer membrane

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis