Rabbit A1 Polyclonal Antibody | anti-A1 antibody

A1 Antibody

Cat. Num. AAA840386

• Gene Names: BCL2A1; GRS; ACC1; ACC2; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: A1; Polyclonal Antibody; A1 Antibody; Bfl-1; anti-A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) protein A purified rabbit anti-A1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 163

• Applicable Applications for anti-A1 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The A1 antibody detects A1 (Bfl-1) of human, mouse, and rat origins.
• Immunogen: Synthetic peptide surrounding amino acid 37 of human A1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-A1 antibody

Background: A1 (also designated Bfl-1) is a Bcl-2 family protein that dimerizes with both Bcl-2 and Bax, and has been identified as a hematopoietic-specific, early inducible gene. Althomugh A1 demonstrated properties similar to those of Bcl-2, its function may be more temporally regulated during myeloid differentiation and is dependent on additional growth stimuli to confer its life promoting properties. A1 is abundantly expressed in bone marrow and at low levels in other tissues. A1 expression has been correlated to stomach cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 168480072
• NCBI GeneID: 597
• NCBI Accession #: NP_001108207.1
• NCBI GenBank Nucleotide #: NM_001114735.1
• UniProt Accession #: Q16548; Q6FGZ4; Q6FH19; Q86W13; Q99524
• Molecular Weight: 18,746 Da
• NCBI Official Full Name: bcl-2-related protein A1 isoform 2
• NCBI Official Synonym Full Names: BCL2-related protein A1
• NCBI Official Symbol: BCL2A1
• NCBI Official Synonym Symbols: GRS; ACC1; ACC2; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• NCBI Protein Information: bcl-2-related protein A1
• UniProt Protein Name: Bcl-2-related protein A1
• Protein Family: A1-specific pheromone
• UniProt Gene Name: BCL2A1
• UniProt Synonym Gene Names: BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5
• UniProt Entry Name: B2LA1_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: mitochondrial outer membrane

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis