BCL2A1 recombinant protein

Bcl2A1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120353

• Gene Names: BCL2A1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Bcl2A1; Bcl2A1 Protein; Human; Recombinant (His Tag); Human Bcl2A1/Bfl-1 protein (His tag); ACC-1 Protein; ACC-2 Protein; ACC1 Protein; ACC2 Protein; BCL2L5 Protein; BFL1 Protein; GRS Protein; HBPA1 Protein; BCL2 related protein A1; BCL2A1 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser152

• Species: Human
• Predicted N Terminal: Met 1
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human BCL2A1 (NP_004040)(Met1-Ser152) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for BCL2A1 recombinant protein

Background: B-cell lymphoma 2-related protein A1 (BCL2A1) is a member of the BCL-2 family of anti-apoptotic proteins that confers resistance to treatment with anti-cancer drugs. Immunohistochemical expression of Wnt11 and BCL2A1 in complete moles and normal villi. Bcl2 family proteins control mitochondrial apoptosis and its members exert critical cell type and differentiation stage-specific functions, acting as barriers against autoimmunity or transformation. Anti-apoptotic Bcl2a1/Bfl1/A1 is frequently deregulated in different types of blood cancers in humans but its physiological role is poorly understood as quadruplication of the Bcl2a1 gene locus in mice hampers conventional gene targeting strategies. In a physiological context, BCL2A1 is mainly expressed in the hematopoietic system, where it facilitates survival of selected leukocytes subsets and inflammation. However, BCL2A1 is overexpressed in a variety of cancer cells, including hematological malignancies and solid tumors, and may contribute to tumor progression. The development of small molecule inhibitors of BCL2A1 may be a promising approach mainly to sensitize tumor cells for apoptosis and thus improve the efficiency of anti-cancer therapy.

Product Categories/Family for BCL2A1 recombinant protein

B Cell Receptor Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 4757840
• NCBI GeneID: 597
• NCBI Accession #: NP_004040
• NCBI GenBank Nucleotide #: NM_004049.3
• UniProt Accession #: Q16548; Q6FGZ4; Q6FH19; Q86W13; Q99524
• Molecular Weight: 18,746 Da
• NCBI Official Full Name: bcl-2-related protein A1 isoform 1
• NCBI Official Synonym Full Names: BCL2-related protein A1
• NCBI Official Symbol: BCL2A1
• NCBI Official Synonym Symbols: GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• NCBI Protein Information: bcl-2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein
• UniProt Protein Name: Bcl-2-related protein A1
• Protein Family: Bcl-2-related protein
• UniProt Gene Name: BCL2A1
• UniProt Synonym Gene Names: BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5
• UniProt Entry Name: B2LA1_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: mitochondrial outer membrane

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis