Western Blot (WB)
(Western Blot: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.)
Rabbit anti-Human Beclin 1 Polyclonal Antibody | anti-BECN1 antibody
Polyclonal Antibody to Beclin 1 (BECN1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BECN1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BECN1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate;;Lane 2: BECN1 knockout MCF7 cell lysate;;Predicted MW: 52kDa ;Observed MW: 60kDa;Primary Ab: 3ug/ml Rabbit Anti-Human BECN1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate;;Lane 2: BECN1 knockout MCF7 cell lysate;;Predicted MW: 52kDa ;Observed MW: 60kDa;Primary Ab: 3ug/ml Rabbit Anti-Human BECN1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 17q21.31
Cellular Component: autophagic vacuole; cytosol; dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; endosome membrane; extrinsic component of membrane; mitochondrial membrane; mitochondrion; nucleus; phagocytic vesicle; protein complex; trans-Golgi network
Molecular Function: GTPase binding; phosphoinositide 3-kinase binding; protein binding; ubiquitin protein ligase binding
Biological Process: aging; apoptosis; autophagosome assembly; autophagy; beta-amyloid metabolic process; cellular defense response; cellular response to amino acid starvation; cellular response to glucose starvation; cellular response to nitrogen starvation; CVT pathway; cytokinesis; defense response to virus; early endosome to late endosome transport; engulfment of apoptotic cell; late endosome to vacuole transport; lysosome organization and biogenesis; macroautophagy; macromitophagy; mitochondrion degradation; mitotic metaphase plate congression; negative regulation of apoptosis; negative regulation of cell proliferation; neuron development; positive regulation of phosphoinositide 3-kinase cascade; protein deubiquitination; receptor catabolic process; regulation of catalytic activity; regulation of cytokinesis; response to drug; response to hypoxia; response to iron(II) ion; response to lead ion; response to vitamin E; viral process
Research Articles on BECN1
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Product Notes
The BECN1 becn1 (Catalog #AAA2028535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Beclin 1 (BECN1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MEG SKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ. It is sometimes possible for the material contained within the vial of "Beclin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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