Rabbit anti-Human BEAN Polyclonal Antibody | anti-BEAN antibody

BEAN, NT (BEAN1, Protein BEAN1, Brain-expressed protein associating with Nedd4 homolog) (Azide free) (HRP)

Cat. Num. AAA6278102

• Gene Names: BEAN1; BEAN; SCA31
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BEAN; Polyclonal Antibody; NT (BEAN1; Protein BEAN1; Brain-expressed protein associating with Nedd4 homolog) (Azide free) (HRP); BEAN1; Brain-expressed protein associating with Nedd4 homolog; anti-BEAN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BEAN antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BEAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human BEAN.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BEAN Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BEAN antibody detected the BEAN protein (arrow).)

Immunohistochemistry (IHC)

(BEAN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BEAN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BEAN antibody

The protein encoded by this gene is one of several proteins that interact with NEDD4, a member of a family of ubiquitin-protein ligases. These proteins have PY motifs in common that bind to the WW domains of NEDD4. NEDD4 is developmentally regulated, and is highly expressed in embryonic tissues. Mutations in this gene (i.e., intronic insertions of >100 copies of pentanucleotide repeats including a (TGGAA)n sequence) are associated with spinocerebellar ataxia type 31. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-BEAN antibody

Antibodies; Proteins

References

Sato, N., et al. Am. J. Hum. Genet. 85(5):544-557(2009). Jolliffe, C.N., et al. Biochem. J. 351 PT 3, 557-565 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 209870062
• NCBI GeneID: 146227
• NCBI Accession #: NP_001129578.1; NP_001171491.1
• NCBI GenBank Nucleotide #: NM_001136106.4
• UniProt Accession #: Q3B7T3; B3KPC0; H3BP97
• Molecular Weight: 22,345 Da
• NCBI Official Full Name: protein BEAN1 isoform 2
• NCBI Official Synonym Full Names: brain expressed, associated with NEDD4, 1
• NCBI Official Symbol: BEAN1
• NCBI Official Synonym Symbols: BEAN; SCA31
• NCBI Protein Information: protein BEAN1
• UniProt Protein Name: Protein BEAN1
• UniProt Gene Name: BEAN1
• UniProt Synonym Gene Names: BEAN
• UniProt Entry Name: BEAN1_HUMAN

NCBI Description

The protein encoded by this gene is one of several proteins that interact with NEDD4, a member of a family of ubiquitin-protein ligases. These proteins have PY motifs in common that bind to the WW domains of NEDD4. NEDD4 is developmentally regulated, and is highly expressed in embryonic tissues. Mutations in this gene (i.e., intronic insertions of >100 copies of pentanucleotide repeats including a (TGGAA)n sequence) are associated with spinocerebellar ataxia type 31. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

BEAN: Defects in BEAN1 are the cause of spinocerebellar ataxia type 31 (SCA31); also known as spinocerebellar ataxia 16q22-linked. A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA31 belongs to the autosomal dominant cerebellar ataxias type III (ADCA III) which are characterized by pure cerebellar ataxia without additional signs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: integral to membrane

Disease: Spinocerebellar Ataxia 31