Rabbit anti-Human, Mouse BDNF Polyclonal Antibody | anti-BDNF antibody

Rabbit Anti-Human BDNF Polyclonal

Cat. Num. AAA807632

• Gene Names: BDNF; ANON2; BULN2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: BDNF; Polyclonal Antibody; Rabbit Anti-Human BDNF Polyclonal; Abrineurin Antibody; ANON2 Antibody; BDNF_Human Antibody; Brain Dervied Neurotrophic Factor Antibody; MGC34632 Antibody; BDNF Antibody: RPE ; BDNF Antibody; anti-BDNF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 247

• Applicable Applications for anti-BDNF antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Synthetic peptide from the N-terminal of human BDNF
• Immunogen Species: Human
• COA: A 1:1000 dilution was sufficient for detection of BDNF on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Conjugate: RPE
• Cellular Localization: Secreted
• Preparation and Storage: Store at -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.)

Western Blot (WB)

(Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Lane 3: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.)

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.)

Related Product Information for anti-BDNF antibody

Brain-derived neurotrophic factor, also known as BDNF, is a protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. Neurotrophic factors are found in the brain and the periphery. The effects of BDNF on motor neurons might be useful in treating patients with motor neuropathies and ALS (1-3).

Product Categories/Family for anti-BDNF antibody

Neuroscience; Neurodegeneration; Alzheimer's Disease; Cardiovascular System; Atherosclerosis; ALS Disease

References

1. Wetmore C., Ernfors P., Persson H., Olson L. (1990) Exp. Neurol. 109(2): 141-152. 2. Thoenen H., Zafra F., Hengerer B., Lindholm D. (1991) Ann NY Acad Sci. 640: 86-90. 3. Chun H.S., Son J.J., Son J.H. (2000) Neuroreport. 11(3): 511-514.

NCBI and Uniprot Product Information

• NCBI GI #: 219842288
• NCBI GeneID: 627
• NCBI Accession #: NP_001137277.1
• NCBI GenBank Nucleotide #: NM_001143805.1
• UniProt Accession #: P23560; Q598Q1; Q6DN19; Q6YNR2; Q6YNR3; Q9BYY7; Q9UC24; A7LA85; A7LA92; D3DQZ2
• Molecular Weight: Predicted molecular weight at ~27.9kDa.
• NCBI Official Full Name: brain-derived neurotrophic factor isoform a preproprotein
• NCBI Official Synonym Full Names: brain-derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Official Synonym Symbols: ANON2; BULN2
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF
• UniProt Entry Name: BDNF_HUMAN

NCBI Description

This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]

Uniprot Description

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; extracellular region; perinuclear region of cytoplasm

Molecular Function: growth factor activity; neurotrophin TRKB receptor binding

Biological Process: axon extension; axon guidance; axon target recognition; behavioral fear response; brain-derived neurotrophic factor receptor signaling pathway; cell-cell signaling; collateral sprouting; dendrite development; feeding behavior; gamma-aminobutyric acid signaling pathway; glutamate secretion; inner ear development; learning and/or memory; mechanoreceptor differentiation; negative regulation of neuroblast proliferation; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; nerve development; nervous system development; neuron recognition; positive regulation of brain-derived neurotrophic factor receptor signaling pathway; positive regulation of collateral sprouting; positive regulation of synaptogenesis; regulation of inhibitory postsynaptic membrane potential; regulation of metabolic process; regulation of retinal cell programmed cell death; regulation of synaptic plasticity; response to drug; synaptogenesis; ureteric bud development

Disease: Bulimia Nervosa, Susceptibility To, 1; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital; Obsessive-compulsive Disorder