Human laminin, alpha 3 ELISA Kit | LAMA3 elisa kit

Human Laminin subunit alpha-3, LAMA3 ELISA Kit

Cat. Num. AAA931822

• Gene Names: LAMA3; E170; LOCS; BM600; LAMNA; lama3a
• Reactivity: Human
• Synonyms: laminin; alpha 3; Human Laminin subunit alpha-3; LAMA3 ELISA Kit; Human Laminin subunit alpha-3 (LAMA3) ELISA kit; BM600; E170; LAMNA; LOCS; lama3a; BM600 150kD subunit; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; kalinin 165kD subunit; laminin alpha 3 subunit; alpha 3 (nicein (150kD); kalinin (165; LAMA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LAMA3. No significant cross-reactivity or interference between human LAMA3 and analogues was observed.
• Sequence Length: 1724

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 9.4 ng/ml-600 ng/ml
• Sensitivity: < 2.35 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMA3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 38045908
• NCBI GeneID: 3909
• NCBI Accession #: NP_000218.2
• NCBI GenBank Nucleotide #: NM_000227.3
• UniProt Accession #: Q16787; Q13679; Q13680; Q6VU67; Q6VU68; Q6VU69; Q76E14; Q96TG0; B0YJ33
• Molecular Weight: 366,649 Da
• NCBI Official Full Name: laminin subunit alpha-3 isoform 2
• NCBI Official Synonym Full Names: laminin, alpha 3
• NCBI Official Symbol: LAMA3
• NCBI Official Synonym Symbols: E170; LOCS; BM600; LAMNA; lama3a
• NCBI Protein Information: laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)
• UniProt Protein Name: Laminin subunit alpha-3
• Protein Family: Laminin
• UniProt Gene Name: LAMA3
• UniProt Synonym Gene Names: LAMNA; E170
• UniProt Entry Name: LAMA3_HUMAN

NCBI Description

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane

Molecular Function: structural molecule activity; receptor binding

Biological Process: regulation of cell adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; hemidesmosome assembly; epidermis development; regulation of embryonic development; cell adhesion; regulation of cell migration

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type