Human TP53BP1 (Tumor Protein p53 Binding Protein 1) ELISA Kit | TP53BP1 elisa kit

Human TP53BP1 (Tumor Protein p53 Binding Protein 1) ELISA Kit

Cat. Num. AAA7606555

• Gene Names: TP53BP1; TP53; p202; 53BP1; TDRD30; p53BP1
• Reactivity: Human
• Synonyms: TP53BP1 (Tumor Protein p53 Binding Protein 1); Human TP53BP1 (Tumor Protein p53 Binding Protein 1) ELISA Kit; TP53BP1 elisa kit

• Reactivity: Human
• Sequence Length: 1972

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP53BP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TP53BP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TP53BP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TP53BP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TP53BP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 85566841
• NCBI GeneID: 7158
• Molecular Weight: 213,989 Da
• NCBI Official Full Name: Tumor protein p53 binding protein 1
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: TP53; p202; 53BP1; TDRD30; p53BP1
• NCBI Protein Information: TP53-binding protein 1; tumor suppressor p53-binding protein 1
• UniProt Protein Name: TP53-binding protein 1
• Protein Family: Tumor suppressor p53-binding protein
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: p53BP1

Uniprot Description

53BP1: a DNA damage checkpoint protein that interacts with HDAC4 protein to mediate the DNA damage response. Inhibits E2F1-mediated apoptosis in prostate cancer cells. Recruited to double strand breaks via the binding of its Tudor domain to dimethylated H3 K20. Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Binds to the central domain of p53. Interacts with Artemis. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on S139. Interacts with histone H4 that has been dimethylated at K20. Has low affinity for histone H4 containing monomethylated K20. Does not bind histone H4 containing unmethylated or trimethylated K20. Has low affinity for histone H3 that has been dimethylated on K79. Does not bind unmethylated histone H3. Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Two alternatively spliced human isoforms have been observed.

Protein type: DNA repair, damage; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: chromosome, telomeric region; cytoplasm; nuclear body; nucleoplasm; nucleus

Molecular Function: methylated histone residue binding; p53 binding; protein binding

Biological Process: DNA damage checkpoint; double-strand break repair via nonhomologous end joining; positive regulation of isotype switching; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein homooligomerization; protein sumoylation; response to DNA damage stimulus