Rabbit anti-Human, Mouse BD3 Polyclonal Antibody | anti-Defb3 antibody

BD3 (beta-Defensin 3)

Cat. Num. AAA621873

• Gene Names: Defb3; BD-3; MGC129397
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BD3; Polyclonal Antibody; BD3 (beta-Defensin 3); Anti -BD3 (beta-Defensin 3); anti-Defb3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes both mouse and human defensin-3, but not other beta-defensins.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.

• Applicable Applications for anti-Defb3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.; Western Blot: 1-10ug/ml using ECL technique.; Optimal dilution determined by the researcher.
• Immunogen: A 13aa peptide mapping at the N-terminus of mature mouse defensin-3 and a 15aa peptide mapping at the N-terminus of mature human defensin-3. The two peptides were mixed and coupled to KLH prior to immunization.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Defb3 antibody

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human a- and b-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a- and b- based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologs of the human beta-defensin have also been identified.

Product Categories/Family for anti-Defb3 antibody

Antibodies; Abs to Defensin

NCBI and Uniprot Product Information

• NCBI GI #: 7304997
• NCBI GeneID: 27358
• NCBI Accession #: NP_038784.1
• NCBI GenBank Nucleotide #: NM_013756.2
• UniProt Accession #: Q9WTL0; Q29R78
• Molecular Weight: 7,126 Da
• NCBI Official Full Name: beta-defensin 3
• NCBI Official Synonym Full Names: defensin beta 3
• NCBI Official Symbol: Defb3
• NCBI Official Synonym Symbols: BD-3; MGC129397
• NCBI Protein Information: beta-defensin 3; OTTMUSP00000022575
• UniProt Protein Name: Beta-defensin 3
• Protein Family: Beta-defensin
• UniProt Gene Name: Defb3
• UniProt Synonym Gene Names: Bd3
• UniProt Entry Name: DEFB3_MOUSE

Uniprot Description

Function: Antimicrobial activity against Gram-negative bacteria E.coli and P.aeruginosa. Ref.1

Subcellular location: Secreted.

Tissue specificity: Highest expression in salivary glands, epididymis, ovary and pancreas and to a lesser extent in lung, liver and brain. Low or no expression in skeletal muscle and tongue. Ref.1 Ref.3

Induction: By bacterial infection. Ref.1

Sequence similarities: Belongs to the beta-defensin family. LAP/TAP subfamily.