Rabbit anti-Human, Mouse TOLLIP Polyclonal Antibody | anti-TOLLIP antibody

TOLLIP (Toll Interacting Protein, FLJ33531, IL-1RAcPIP) (HRP)

Cat. Num. AAA6396889

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TOLLIP; Polyclonal Antibody; TOLLIP (Toll Interacting Protein; FLJ33531; IL-1RAcPIP) (HRP); anti-TOLLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TOLLIP. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TOLLIP antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TOLLIP, aa1-274 (NP_061882.2).
• Immunogen Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TOLLIP rabbit polyclonal antibody. Western Blot analysis of TOLLIP expression in mouse brain.)

Western Blot (WB)

(TOLLIP rabbit polyclonal antibody. Western Blot analysis of TOLLIP expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of TOLLIP expression in transfected 293T cell line by TOLLIP polyclonal antibody. Lane 1: TOLLIP transfected lysate (30.3kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-TOLLIP antibody

Antibodies; Toll Like Receptors (TLR)

NCBI and Uniprot Product Information

• NCBI GI #: 21361619
• NCBI GeneID: 54472
• NCBI Accession #: NP_061882.2
• NCBI GenBank Nucleotide #: NM_019009
• UniProt Accession #: Q9H0E2; Q9H9E6; Q9UJ69
• Molecular Weight: 30,282 Da
• NCBI Official Full Name: toll-interacting protein
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein; adapter protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: TOLLIP
• UniProt Entry Name: TOLIP_HUMAN

NCBI Description

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [provided by RefSeq, Aug 2011]

Uniprot Description

TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.

Protein type: Inhibitor; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nuclear body; interleukin-1 receptor complex; perinuclear region of cytoplasm; cytoplasm; interleukin-18 receptor complex; cytosol

Molecular Function: protein binding; signal transducer activity; SUMO binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; interleukin-1, Type I receptor binding; kinase binding

Biological Process: positive regulation of protein sumoylation; cell-cell signaling; epithelial cell differentiation; autophagy; innate immune response; signal transduction; inflammatory response; phosphorylation; leukocyte activation