Rabbit BCL9L Polyclonal Antibody | anti-BCL9L antibody
Anti-BCL9L Antibody
Gene Names
BCL9L; B9L; BCL9-2; DLNB11
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Immunogen Affinity Purified
Synonyms
BCL9L; Polyclonal Antibody; Anti-BCL9L Antibody; B-cell CLL/lymphoma 9-like protein; BCL9 like; B-cell lymphoma 9-like protein; BCL9-like protein; Protein BCL9-2; DLNB11; anti-BCL9L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
1499
Applicable Applications for anti-BCL9L antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
Immunogen
E Coli-derived human BCL9L recombinant protein (Position: E397-A666).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
Relevant Detection Systems
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
Relevant Detection Systems
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P).
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-BCL9L antibody
Description: Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.
Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.
Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.
References
1. Brembeck, F. H., Schwarz-Romond, T., Bakkers, J., Wilhelm, S., Hammerschmidt, M., Birchmeier, W. Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions. Genes Dev. 18: 2225-2230, 2004. 2. Katoh, M., Katoh, M. Identification and characterization of human BCL9L gene and mouse Bcl9l gene in silico. Int. J. Molec. Med. 12: 643-649, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
B-cell CLL/lymphoma 9-like protein
NCBI Official Synonym Full Names
BCL9 like
NCBI Official Symbol
BCL9L
NCBI Official Synonym Symbols
B9L; BCL9-2; DLNB11
NCBI Protein Information
B-cell CLL/lymphoma 9-like protein
UniProt Protein Name
B-cell CLL/lymphoma 9-like protein
Protein Family
UniProt Gene Name
BCL9L
UniProt Synonym Gene Names
DLNB11; B-cell lymphoma 9-like protein
UniProt Entry Name
BCL9L_HUMAN
Uniprot Description
Bcl-9L: a novel beta-catenin-interacting protein that translocates beta-catenin to the nucleus, enhancing beta-catenin mediated transcription. Can play an important role in colorectal carcinogenesis. Nuclear expression of Bcl-9L is closely associated with the high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: transcription coactivator activity; beta-catenin binding
Biological Process: transcription, DNA-dependent; somatic stem cell maintenance; regulation of cell morphogenesis; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on BCL9L
Similar Products
Product Notes
The BCL9L bcl9l (Catalog #AAA1752524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCL9L Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCL9L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA). WB: 0.1-0.5ug/ml IHC-P: 0.5-1ug/ml Direct ELISA: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the BCL9L bcl9l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL9L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
