Rabbit anti-Rat Bcl2 Associated X Protein (Bax) Polyclonal Antibody | anti-Bax antibody

Polyclonal Antibody to Bcl2 Associated X Protein (Bax)

Cat. Num. AAA2090513

• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Bcl2 Associated X Protein (Bax); Polyclonal Antibody; Polyclonal Antibody to Bcl2 Associated X Protein (Bax); BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4; anti-Bax antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against Bax. It has been selected for its ability to recognize Bax in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-Bax antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-2ug/mL; 1:270-1000; Immunohistochemistry: 5-20ug/mL; 1:27-100; Immunocytochemistry: 5-20ug/mL; 1:27-100
• Immunogen: Recombinant Bcl2 Associated X Protein (Bax)
• Cross Reactivity: Rat
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant Bax, Rat.)

NCBI and Uniprot Product Information

• NCBI GI #: 41386764
• NCBI GeneID: 280730
• NCBI Accession #: NP_776319.1
• NCBI GenBank Nucleotide #: NM_173894.1
• UniProt Accession #: O02703
• Molecular Weight: – Da
• NCBI Official Full Name: apoptosis regulator BAX
• NCBI Official Synonym Full Names: BCL2 associated X, apoptosis regulator
• NCBI Official Symbol: BAX
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Protein
• UniProt Gene Name: BAX

Uniprot Description

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production (). Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria ().