Typical Testing Data/Standard Curve (for reference only)
IGFBP-1 elisa kit
IGFBP-1 (Mouse) ELISA Kit
Gene Names
IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Synonyms
IGFBP-1; IGFBP-1 (Mouse) ELISA Kit; Insulin-like growth factor-binding protein 1; Placental protein 12; PP12; IGFBP-1 elisa kit
Specificity
Detects mouse IFNgamma in direct ELISAs and Western-blots. This antibody does not crossreact with recombinant human(rh) IFNgamma, rrIFNgamma, rpIFNgamma, rfeIFNgamma, or rcrIFNgamma.
Sequence Length
259
Samples
Mouse Serum, Mouse Plasma, Cell Lysate, Culture Supernatants And Buffered Solution
Assay Type
Quantitative Sandwich
Sensitivity
0.831 pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IGFBP-1 elisa kit
Intended Uses: The Mouse IFNgamma ELISA kit is to be used for the determination of Mouse IFNgamma in Mouse serum, Mouse plasma, cell lysate, culture supernatants and buffered solution. The assay will recognize native and recombinant Mouse IFNgamma.
Principle of the Assay: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to Mouse IFNgamma. Samples are pipetted into these wells. Nonbound Mouse IFNgamma and other components of the sample should be removed by washing, then monoclonal antibody specific to Mouse IFNgamma added. In order to quantitatively determine the amount of Mouse IFNgamma present in the sample, Streptavidin Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Mouse IFNgamma!!Background/Introduction: Interferon gamma (IFNgamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was recognized in 1970 when tuberculin-sensitized peritoneal cells were challenged with PPD and resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. That report also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for TB. This interferon was later called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFNgamma belongs. In humans, the IFNgamma protein is encoded by the IFNG gene. IFNgamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNgamma is an important activator of macrophages. Aberrant IFNgamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNgamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNgamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Principle of the Assay: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to Mouse IFNgamma. Samples are pipetted into these wells. Nonbound Mouse IFNgamma and other components of the sample should be removed by washing, then monoclonal antibody specific to Mouse IFNgamma added. In order to quantitatively determine the amount of Mouse IFNgamma present in the sample, Streptavidin Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Mouse IFNgamma!!Background/Introduction: Interferon gamma (IFNgamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was recognized in 1970 when tuberculin-sensitized peritoneal cells were challenged with PPD and resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. That report also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for TB. This interferon was later called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFNgamma belongs. In humans, the IFNgamma protein is encoded by the IFNG gene. IFNgamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNgamma is an important activator of macrophages. Aberrant IFNgamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNgamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNgamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Product Categories/Family for IGFBP-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,904 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 1
NCBI Official Synonym Full Names
insulin like growth factor binding protein 1
NCBI Official Symbol
IGFBP1
NCBI Official Synonym Symbols
AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
NCBI Protein Information
insulin-like growth factor-binding protein 1
UniProt Protein Name
Insulin-like growth factor-binding protein 1
UniProt Gene Name
IGFBP1
UniProt Synonym Gene Names
IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
UniProt Entry Name
IBP1_HUMAN
NCBI Description
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
Uniprot Description
IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7p12.3
Cellular Component: extracellular region; extracellular space
Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding
Biological Process: cellular protein metabolic process; insulin receptor signaling pathway; positive regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; tissue regeneration; unfolded protein response
Research Articles on IGFBP-1
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Product Notes
The IGFBP-1 igfbp1 (Catalog #AAA495268) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IGFBP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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