Rabbit anti-Human, Rat TAF1A Polyclonal Antibody | anti-TAF1A antibody

TAF1A Antibody

Cat. Num. AAA9604753

• Gene Names: TAF1A; SL1; RAFI48; TAFI48; MGC:17061
• Reactivity: Human, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TAF1A; Polyclonal Antibody; TAF1A Antibody; MGC:17061; RAFI48; RNA polymerase I-specific TBP-associated factor 48 kDa; SL1 48kD subunit; SL1; Taf1a; TAF1A_HUMAN; TAFI48; TATA box binding protein (TBP) associated factor RNA polymerase I; A 48kDa; TATA box binding protein (Tbp)-associated factor; RNA polymerase I; A; TATA box binding protein associated factor 1A; TATA box binding protein associated factor RNA polymerase I subunit A; TATA box-binding protein-associated factor 1A; TATA box-binding protein-associated factor RNA polymerase I subunit A; TBP associated factor RNA polymerase I 48 kDa; TBP-associated factor 1A; Transcription factor SL1; Transcription initiation factor SL1/TIF-IB subunit A; anti-TAF1A antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAF1A antibody detects endogenous levels of total TAF1A
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-TAF1A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit
• Similarity: Pig (91%), Bovine (82%), Horse (91%), Sheep (82%), Rabbit (91%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9604753 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using TAF1A antibody.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-TAF1A antibody

Function: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
Subunit Structure: Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1B. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with UBFT. Interacts with CEBPA (isoform 1 and isoform 4) (PubMed:20075868).

NCBI and Uniprot Product Information

• NCBI GI #: 319996599
• NCBI GeneID: 9015
• NCBI Accession #: NP_001188465.1
• NCBI GenBank Nucleotide #: NM_001201536.1
• UniProt Accession #: Q15573; Q9NWA1; B2RDZ8; D3DTB7
• Molecular Weight: Observed: 53 kD; Predicted: 53 kDa
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit A isoform 1
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor, RNA polymerase I subunit A
• NCBI Official Symbol: TAF1A
• NCBI Official Synonym Symbols: SL1; RAFI48; TAFI48; MGC:17061
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit A
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit A
• Protein Family: TATA box-binding protein-associated factor
• UniProt Gene Name: TAF1A
• UniProt Synonym Gene Names: TAFI48; TBP-associated factor 1A

NCBI Description

This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011]

Uniprot Description

Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.