Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit | Bcl11A elisa kit

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

Cat. Num. AAA2705529

• Gene Names: BCL11A; EVI9; CTIP1; DILOS; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• Reactivity: Human
• Synonyms: B-Cell CLL/Lymphoma 11A (Bcl11A); Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit; BCL11A-L; BCL11A-S; BCL11A-XL; CTIP1; EVI9; Ecotropic Viral Integration Site 9; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 homolog; Zinc finger protein 856; Bcl11A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 11A (Bcl11A).; No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 11A (Bcl11A) and analogues was observed.
• Sequence Length: 142

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bcl11A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 11A (Bcl11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 11A (Bcl11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20336305
• NCBI GeneID: 53335
• NCBI Accession #: NP_075044.2
• NCBI GenBank Nucleotide #: NM_022893.3
• UniProt Accession #: Q9H165; Q66LN6; Q86W14; Q8WU92; Q96JL6; Q9H163; Q9H164; Q9H3G9; Q9NWA7; D6W5D7
• Molecular Weight: 15,720 Da
• NCBI Official Full Name: B-cell lymphoma/leukemia 11A isoform 1
• NCBI Official Synonym Full Names: B cell CLL/lymphoma 11A
• NCBI Official Symbol: BCL11A
• NCBI Official Synonym Symbols: EVI9; CTIP1; DILOS; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• NCBI Protein Information: B-cell lymphoma/leukemia 11A
• UniProt Protein Name: B-cell lymphoma/leukemia 11A
• Protein Family: B-cell lymphoma/leukemia
• UniProt Gene Name: BCL11A
• UniProt Synonym Gene Names: CTIP1; EVI9; KIAA1809; ZNF856; BCL-11A; EVI-9

NCBI Description

This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor associated with the BAF SWI/SNF chromatin remodeling complex (). Repressor of fetal hemoglobin (HbF) level (PubMed:26375765). Involved in brain development (PubMed:27453576). Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 ().