Testing Data
Rabbit anti-Human Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody
Anti-Human Bcl-2 Antibody
Reactivity
Human
Applications
Western Blot
Purity
Protein A Purified
Synonyms
Bcl-2; Polyclonal Antibody; Anti-Human Bcl-2 Antibody; Human Bcl-2; anti-Bcl-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This purified IgG antibody was prepared from monospecific rabbit antiserum by Protein A chormatography. The antibody is directed against human Bcl-2 and is useful in determining its presence in various assays.
Purity/Purification
Protein A Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
154
Applicable Applications for anti-Bcl-2 antibody
Western Blot (WB)
Application Notes
This purified IgG antibody against Human Bcl-2 has been tested for use in immunofluorescence microscopy and immunoblotting. The antibody recognizes a 27 kDa band corresponding to human bcl-2. Anti-Bcl-2 shows perinuclear staining of Bcl-2. Reactivity in other immunoassays is unknown.
Immunogen
This purified IgG antibody was prepared from rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 62-76 (RDPVARTSPLQTPAA) of human Bcl-2.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Bcl-2 antibody
Bcl-2 is a human proto-oncogene located chromosome 18. Its product is an integral membrane protein (also called Bcl-2) located in the membranes of the endoplasmic reticulum (ER), nuclear envelope, and in the outer membranes of the mitochondria. The gene was discovered as the translocated locus in a B-cell leukemia (hence the name). This translocation is also found in some B-cell lymphomas. In the cancerous B cells, the portion of chromosome 18 containing the BCL-2 locus has undergone a reciprocal translocation with the portion of chromosome 14 containing the antibody heavy chain locus. This t(14;18) translocation places the BCL-2 gene close to the heavy chain gene enhancer. This enhancer is very active in B cells and therefore results in high levels of Bcl-2 expression in these cells. High levels of the Bcl-2 protein protect the cells from early death by apoptosis. The Bcl-2 protein suppresses apoptosis by preventing the activation of the caspases that carry out the process. It is conceived that introduction of the Bcl-2 gene into the cells of injured tissue will reduce cell death and improve the clinical outcome of the injury
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Bcl-2 (Catalog #AAA4157818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human Bcl-2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). This purified IgG antibody against Human Bcl-2 has been tested for use in immunofluorescence microscopy and immunoblotting. The antibody recognizes a 27 kDa band corresponding to human bcl-2. Anti-Bcl-2 shows perinuclear staining of Bcl-2. Reactivity in other immunoassays is unknown. Researchers should empirically determine the suitability of the Bcl-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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