Testing Data
Rabbit anti-Human COX-2 Polyclonal Antibody | anti-COX-2 antibody
Anti-Human COX-2 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified
Synonyms
COX-2; Polyclonal Antibody; Anti-Human COX-2 Antibody; Human COX-2; anti-COX-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antiserum is directed against human Cox-2 and is useful in determining its presence in western blotting and ELISA experiments. The antibody does not cross react with Cox-1. Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation. This antibody can detect Cox-2 in human, mouse and rat. Reactivity of this antibody with Cox-2 from other species is unknown.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
608
Applicable Applications for anti-COX-2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting and ELISA. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a GST fusion protein, the epitope of which maps to the carboxy-terminus of human Cox-2.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-COX-2 antibody
COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a "super aspirin" and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
COX-2
Similar Products
Product Notes
The COX-2 (Catalog #AAA4158671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human COX-2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COX-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting and ELISA. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown. Researchers should empirically determine the suitability of the COX-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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