Rabbit anti-Human, Mouse BCAP31 Polyclonal Antibody | anti-BCAP31 antibody

BCAP31 polyclonal antibody

Cat. Num. AAA3000200

• Gene Names: BCAP31; CDM; BAP31; 6C6-AG; DXS1357E
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BCAP31; Polyclonal Antibody; BCAP31 polyclonal antibody; B-cell receptor-associated protein 31; BCR-associated protein 31; Bap31; 6C6-AG tumor-associated antigen; Protein CDM; p28; BAP31; DXS1357E; anti-BCAP31 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: BCAP31 polyclonal antibody detects endogenous levels of BCAP31 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Applicable Applications for anti-BCAP31 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human BCAP31.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of BCAP31 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysate(40ug)Lane2:HepG2 whole cell lysate(40ug)Lane3:The lung tissue lysate of Mouse(40ug))

Related Product Information for anti-BCAP31 antibody

Background: BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis. During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria. Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway. Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface.

NCBI and Uniprot Product Information

• NCBI GI #: 213511012
• NCBI GeneID: 10134
• NCBI Accession #: NP_001132913.1
• NCBI GenBank Nucleotide #: NM_001139441.1
• UniProt Accession #: P51572; Q13836; Q96CF0; B3KQ79; D3DWV5
• Molecular Weight: 27,992 Da
• NCBI Official Full Name: B-cell receptor-associated protein 31 isoform b
• NCBI Official Synonym Full Names: B-cell receptor-associated protein 31
• NCBI Official Symbol: BCAP31
• NCBI Official Synonym Symbols: CDM; BAP31; 6C6-AG; DXS1357E
• NCBI Protein Information: B-cell receptor-associated protein 31; p28 Bap31; BCR-associated protein Bap31; 6C6-AG tumor-associated antigen
• UniProt Protein Name: B-cell receptor-associated protein 31
• Protein Family: B-cell receptor-associated protein
• UniProt Gene Name: BCAP31
• UniProt Synonym Gene Names: BAP31; DXS1357E; BCR-associated protein 31; Bap31
• UniProt Entry Name: BAP31_HUMAN

NCBI Description

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Uniprot Description

BCAP31 iso2: May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. Homodimer and heterodimer with BCAP29. Binds CASP8 (isoform 9) as a complex containing BCAP31, BCAP29, BCL2 and/or BCL2L1. Interacts with VAMP3, VAMP1 and membrane IgD immunoglobulins. May interact with ACTG1 and non-muscle myosin II. Interacts with PTPLB. Ubiquitous. Belongs to the BCAP29/BCAP31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; clathrin-coated vesicle; membrane; endoplasmic reticulum; integral to plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; MHC class I protein binding; protein complex binding

Biological Process: intracellular protein transport; elevation of cytosolic calcium ion concentration; antigen processing and presentation of peptide antigen via MHC class I; ER to Golgi vesicle-mediated transport; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of caspase activity; spermatogenesis; elevation of mitochondrial calcium ion concentration; cell structure disassembly during apoptosis

Disease: Deafness, Dystonia, And Cerebral Hypomyelination