Rabbit MEF2D Polyclonal Antibody | anti-MEF2D antibody

MEF2D (Phospho-Ser444) Antibody

Cat. Num. AAA9502493

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MEF2D; Polyclonal Antibody; MEF2D (Phospho-Ser444) Antibody; MEFD; Myocyte-specific enhancer factor 2D; anti-MEF2D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: MEF2D (Phospho-Ser444) Antibody detects endogenous levels of MEF2D only when phosphorylated at Ser444.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-MEF2D antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S444; M:S437; R:S430
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEF2D around the phosphorylation site of Ser444.
• Immunogen Range: 410-459
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MEF2D (Phospho-Ser444) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HepG2 cells treated with forskolin 40nM 30', using MEF2D (Phospho-Ser444) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 410442515
• NCBI GeneID: 4209
• NCBI Accession #: NP_001258558.1
• NCBI GenBank Nucleotide #: NM_001271629.1
• UniProt Accession #: Q14814; Q14815; Q5T9U5; Q5T9U6; D3DVC0
• Molecular Weight: 55 kDa
• NCBI Official Full Name: myocyte-specific enhancer factor 2D isoform 2
• NCBI Official Synonym Full Names: myocyte enhancer factor 2D
• NCBI Official Symbol: MEF2D
• NCBI Protein Information: myocyte-specific enhancer factor 2D
• UniProt Protein Name: Myocyte-specific enhancer factor 2D
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2D

NCBI Description

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis ().