Western Blot (WB)
(Sample Data: Western blot analysis of BAX in HL-60 cell lysates with BAX antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Rabbit anti-Human BAX Polyclonal Antibody | anti-BAX antibody
Anti-BAX
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
BAX; Polyclonal Antibody; Anti-BAX; anti-BAX antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the BAX protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-BAX antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
BAX antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human BAX
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of BAX in HL-60 cell lysates with BAX antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of BAX in HL-60 cell lysates with BAX antibody at (A) 1, (B) 2, and (C) 4 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry staining of HL-60 cells using BAX at 2 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry staining of HL-60 cells using BAX at 2 ug/ml.)
Related Product Information for anti-BAX antibody
BAX is a proapoptotic protein of the BCL2 protein family. BAX forms a heterodimer with BCL2 and functions as an apoptotic activator. BAX interacts with and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in the mitochondrial membrane potential and the release of cytochrome c (1). The expression of BAX gene is regulated by the tumor suppressor p53 and BAX has been shown to be involved in p53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene (2).
Product Categories/Family for anti-BAX antibody
References
1. Deveraux Q L, et al: X-linked X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388: 300-304, 1997.
2. Tang G, et al: Inhibition of JNK activation through NF-kappa-B target genes. Nature 414: 313-317, 2001.
2. Tang G, et al: Inhibition of JNK activation through NF-kappa-B target genes. Nature 414: 313-317, 2001.
Similar Products
Product Notes
The BAX (Catalog #AAA515870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BAX reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BAX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the BAX for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
