Rabbit Bassoon Polyclonal Antibody | anti-BSN antibody

Bassoon Antibody

Cat. Num. AAA9612017

• Gene Names: BSN; ZNF231
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Xenopus(86%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Bassoon; Polyclonal Antibody; Bassoon Antibody; Bassoon protein; bsn; BSN_HUMAN; KIAA0434; Neuronal double zinc finger protein; Presynaptic cytomatrix protein; Protein bassoon; Zinc finger protein 231; ZNF 231; ZNF231; anti-BSN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Xenopus(86%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Bassoon Antibody detects endogenous levels of total Bassoon.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 3926

• Applicable Applications for anti-BSN antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Bassoon, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using Bassoon Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612017 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612017 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-BSN antibody

Function: Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity).

Post Translational Modifications: Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization (By similarity).

Subcellular Location: Cytoplasm. Cell junction>Synapse>Presynaptic active zone. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Peripheral membrane protein. Note: In retina, is localized in the outer plexiform layer at ribbon synapses formed by rods and cones but was absent from basal synaptic contacts formed by cones. In the retinal inner plexiform layer localized to conventional inhibitory GABAergic synapses, made by amacrine cells, but absent from the bipolar cell ribbon synapses.

Tissue Specificity: Exclusively expressed in brain.

Subunit Structure: Interacts with PCLO, ERC2/CAST1, RIMS1 and UNC13A (By similarity). Interacts with TPRG1L (By similarity). Interacts with DYNLL1 and DYNLL2; these interactions potentially link PTVs to dynein and myosin V motor complexes (PubMed:19380881). Interacts with ATG5; this interaction is important for the regulation of presynaptic autophagy (By similarity). Interacts (via C-terminus) with TRIO (via N-terminus) (By similarity). Interacts with CTBP1 (By similarity). Interacts with SIAH1; this interaction negatively regulates SIAH1 E3 ligase activity (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 229463040
• NCBI GeneID: 8927
• UniProt Accession #: Q9UPA5
• Molecular Weight: Observed Molecular Weight: (Observed)416kD.; Predicted Molecular Weight: (Calculated)416kDa.
• NCBI Official Full Name: Protein bassoon
• NCBI Official Synonym Full Names: bassoon presynaptic cytomatrix protein
• NCBI Official Symbol: BSN
• NCBI Official Synonym Symbols: ZNF231
• NCBI Protein Information: protein bassoon
• UniProt Protein Name: Protein bassoon
• Protein Family: Protein bassoon
• UniProt Gene Name: BSN
• UniProt Synonym Gene Names: KIAA0434; ZNF231
• UniProt Entry Name: BSN_HUMAN

NCBI Description

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008]

Uniprot Description

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: axon; postsynaptic density; dendrite; presynaptic active zone; excitatory synapse; cell junction; nucleus

Molecular Function: metal ion binding

Biological Process: synaptic transmission