Rabbit anti-Human BarX1 Polyclonal Antibody | anti-BarX1 antibody

BarX1, ID (Homeobox Protein BarH-like 1) (PE)

Cat. Num. AAA6464966

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BarX1; Polyclonal Antibody; ID (Homeobox Protein BarH-like 1) (PE); anti-BarX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BARX1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BarX1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:10-1:50; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human BARX1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BarX1(arrow) using rabbit polyclonal BarX1 Antibody (C-term) (RB14083). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BarX1 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(BarX1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BarX1 antibody detected the BarX1 protein (arrow).)

Product Categories/Family for anti-BarX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Lowry, R.B., Am. J. Med. Genet. A 143(11), 1227-1230 (2007). 2. Gould, D.B., Genomics 68(3), 336-342 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 153218470
• NCBI GeneID: 56033
• NCBI Accession #: NP_067545
• NCBI GenBank Nucleotide #: NM_021570
• UniProt Accession #: Q9HBU1
• Molecular Weight: 27kDa
• NCBI Official Full Name: homeobox protein BarH-like 1
• NCBI Official Synonym Full Names: BARX homeobox 1
• NCBI Official Symbol: BARX1
• NCBI Protein Information: homeobox protein BarH-like 1
• UniProt Protein Name: Homeobox protein BarH-like 1
• Protein Family: Homeobox protein
• UniProt Gene Name: BARX1
• UniProt Entry Name: BARX1_HUMAN

NCBI Description

This gene encodes a member of the Bar subclass of homeobox transcription factors. Studies of the mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. The protein may also be associated with differentiation of stomach epithelia. [provided by RefSeq, Jul 2008]

Uniprot Description

BARX1: Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity. Binds to a regulatory module of the NCAM promoter. Belongs to the BAR homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q12

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; spleen development; negative regulation of Wnt receptor signaling pathway; epithelial cell differentiation; transcription, DNA-dependent; cell-cell signaling; regulation of transcription, DNA-dependent