Rabbit anti-Human BarX1 Polyclonal Antibody | anti-BarX1 antibody

BarX1, CT (Homeobox Protein BarH-like 1) (MaxLight 550)

Cat. Num. AAA6464972

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BarX1; Polyclonal Antibody; CT (Homeobox Protein BarH-like 1) (MaxLight 550); anti-BarX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BarX1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-BarX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human BarX1 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BarX1 Antibody (C-term) in CEM cell line lysates (35ug/lane). BarX1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of BarX1 (arrow) using rabbit polyclonal BarX1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BarX1 gene.)

Product Categories/Family for anti-BarX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Lowry, R.B., Am. J. Med. Genet. A 143(11), 1227-1230 (2007). 2. Gould, D.B., Genomics 68(3), 336-342 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 153218470
• NCBI GeneID: 56033
• NCBI Accession #: NP_067545
• NCBI GenBank Nucleotide #: NM_021570
• UniProt Accession #: Q9HBU1
• Molecular Weight: 27kDa
• NCBI Official Full Name: homeobox protein BarH-like 1
• NCBI Official Synonym Full Names: BARX homeobox 1
• NCBI Official Symbol: BARX1
• NCBI Protein Information: homeobox protein BarH-like 1
• UniProt Protein Name: Homeobox protein BarH-like 1
• Protein Family: Homeobox protein
• UniProt Gene Name: BARX1
• UniProt Entry Name: BARX1_HUMAN

NCBI Description

This gene encodes a member of the Bar subclass of homeobox transcription factors. Studies of the mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. The protein may also be associated with differentiation of stomach epithelia. [provided by RefSeq, Jul 2008]

Uniprot Description

BARX1: Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity. Binds to a regulatory module of the NCAM promoter. Belongs to the BAR homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q12

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; spleen development; negative regulation of Wnt receptor signaling pathway; epithelial cell differentiation; transcription, DNA-dependent; cell-cell signaling; regulation of transcription, DNA-dependent