Rabbit anti-Human BANK1 Polyclonal Antibody | anti-BANK1 antibody

BANK1, ID (B Cell Scaffold Protein With Ankyrin Repeats) (Biotin)

Cat. Num. AAA6464923

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BANK1; Polyclonal Antibody; ID (B Cell Scaffold Protein With Ankyrin Repeats) (Biotin); anti-BANK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BANK1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 755

• Applicable Applications for anti-BANK1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 119-146 in the central region of human BANK1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HepG2 cell line lysates (35ug/lane) usingMBS624841. BANK1 (arrow) was detected usingMBS624841.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS624841 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BANK1 antibody

BANK1 is a B-cell-specific scaffold protein and LYN (MIM 165120) tyrosine kinase substrate that promotes tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors.

Product Categories/Family for anti-BANK1 antibody

Antibodies; Proteins

References

1. Orozco, G., et.al., Arthritis Rheum. 60(2), 372-379 (2009). 2. Guo, L., et.al., Genes Immun. 10(5), 531-538 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 144953889
• NCBI Accession #: NP_001077376.1
• NCBI GenBank Nucleotide #: NP_001077376.1
• UniProt Accession #: Q8NDB2
• Molecular Weight: 89.335
• NCBI Official Full Name: B-cell scaffold protein with ankyrin repeats 1 isoform 2
• UniProt Protein Name: B-cell scaffold protein with ankyrin repeats
• Protein Family: B-cell scaffold protein with ankyrin repeats
• UniProt Gene Name: BANK1
• UniProt Entry Name: BANK1_HUMAN

Uniprot Description

BANK1: Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2. Genetic variations in BANK1 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q24

Biological Process: negative regulation of translational initiation; negative regulation of protein kinase B signaling cascade; positive regulation of MAPKKK cascade; B cell activation; negative regulation of B cell activation

Disease: Systemic Lupus Erythematosus